Use of an Attenuated Strain of Tobamovirus for Early Detection of Virus-Induced Gene Silencing
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概要
- 論文の詳細を見る
Virus-induced gene silencing (VIGS) can be used to study gene function by mediating sequence-specific mRNA degradation and suppressing the expression of endogenous target genes. We previously demonstrated that the TocJ vector based on the tomato mosaic tobamoviruses (ToMV) was able to multiply, spread systemically and express green fluorescence protein in Solanaceous plants. TocJ harbouring fragments of endogenous genes could induce VIGS of the parental gene expression, but also induced viral infection symptoms. In this study, an attenuated strain of ToMV, L11A, was used to construct a ToMV vector in order to reduce the virus-induced symptoms. This new vector, named LcJ, was able to spread systemically and mediated VIGS of endogenous genes without visible symptoms. We propose that the use of this attenuated strain for the construction of virus vectors is beneficial for the induction of VIGS.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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Takizawa Mari
Department of Pediatrics, National Defense Medical College
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Watanabe Yuichiro
Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo
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HORI Koichi
Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo
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Hori K
Department Of Life Sciences The University Of Tokyo
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Takizawa Mari
Department Of Pediatrics St. Marianna University School Of Medicine Yokohama City Seibu Hospital
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Takizawa M
Department Of Life Sciences The University Of Tokyo
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Takizawa M
Aids Research Center National Institute Of Infectious Diseases
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Watanabe Yuichiro
Department Of Life Sciences The University Of Tokyo
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Hori Koichi
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo
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Watanabe Yuichiro
Department Of Life Science Graduate School Of Arts And Science The University Of Tokyo
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Takizawa Mari
Department Of Pediatrics National Defense Medical College
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Takizawa Mari
Department of Life Sciences, the University of Tokyo
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