Quantification and Localization of Fucoidan in Laminaria japonica Using a Novel Antibody
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概要
- 論文の詳細を見る
The establishment of a simple technique to determine the concentration of fucoidan was developed by using a monoclonal antibody against fucoidan. This antibody reacted with fucoidans purified from Laminaria japonica Areschoug (Makombu in Japanese) and Kjellmaniella gyrate Miyabe (Gagome), but not with polysaccharides from Undaria pinnatifida Suringar (Wakame). Neither laminarin nor algenic acid, which are constituents in Laminaria japonica, were recognized by the prepared antibody. Application of the enzymed-linked immunosorbent assay (ELISA) inhibition assay increased the specificity of fucoidan in measuring the fucoidan contents. On the basis of these results, it was ascertained that the ELISA inhibition assay of using the anti-fucoidan monoclonal antibody was rapid, accurate, and sensitive in measuring the content of fucoidan. In addition, the localization of fucoidan in Laminaria japonica was investigated. This is the first report of fucoidan being restricted to the outer cortical layer.
- 社団法人 日本農芸化学会の論文
- 2009-02-23
著者
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Ojima Takao
Graduate School Of Fisheries Science Hokkaido University
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Kanazawa K
Laboratory Of Food And Nutritional Chemistry Graduate School Of Agriculture Kobe University
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Kanazawa Kazuki
Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.
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Kanazawa Kazuki
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Kanazawa K
Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.
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Hashimoto Takashi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Mizuno Masashi
Division Of Science Of Biological Resources The Graduate School Of Science And Technology
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Mizuno Masashi
Division Of Life Science Graduate School Of Science And Technology Kobe University
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Mizuno Masashi
Graduate School Of Science And Technology Kobe University
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Mizuno Masashi
Nagano Japan Radio Co. Ltd. Currently With Kyushu Institute Of Technology
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Hashimoto T
Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.
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Mizuno M
Kobe Univ. Kobe Jpn
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Hashimoto Takashi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kanazawa Kazuki
Graduate School Of Agricultural Sci. Kobe Univ.
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Hashimoto T
Graduate School Of Agricultural Sci. Kobe Univ.
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Mizuno M
Pharmaceutical Research Center Mochida Pharmaceutical Co. Ltd.
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NISHITANI Yosuke
Organization of Advanced Science and Technology, Kobe University
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TANOUE Takeshi
Graduate School of Agricultural Science, Kobe University
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MATOBA Yoshie
Graduate School of Agricultural Science, Kobe University
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Morimoto M
Graduate School Of Science And Technology Kobe University
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Nishitani Yosuke
Organization Of Advanced Science And Technology Kobe University
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Matoba Yoshie
Graduate School Of Agricultural Science Kobe University
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Nishitani Yosuke
Organization Of Advanced Sci. And Technol. Kobe Univ.
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Tanoue Takeshi
Graduate School Of Agricultural Science Kobe University
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Hashimoto Takashi
Graduate School Of Biol. Sci.
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Hashimoto Takashi
Graduate School of Agricultural Science, Kobe University
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Mizuno Masashi
Graduate School of Agricultural Science, Kobe University
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