Tea Catechin Suppresses Adipocyte Differentiation Accompanied by Down-regulation of PPARγ2 and C/EBPα in 3T3-L1 Cells

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概要

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• Obesity is a serious health problem, and its prevention is promoted through life style including diet and exercise. In this study, we investigated the suppressive effects of tea catechin on the differentiation of 3T3-L1 preadipocytes to adipocytes. (−)-Catechin 3-gallate (CG), (−)-epigallocatechin (EGC), (−)-epicatechin 3-gallate, and (−)-epigallocatechin 3-gallate at 5 μM suppressed intracellular lipid accumulation. The suppressive effects of CG and EGC were stronger than the others, and CG and EGC also suppressed the activity of glycerol-3-phosphate dehydrogenase as a differentiation marker. These catechins inhibited the expression of peroxisome proliferator-activated receptor (PPAR) γ2 and CCAAT/enhancer-binding protein (C/EBP) α, both of which act as key transcription factors at an early stage of differentiation, followed by the expression of glucose transporter (GLUT) 4 at a later stage. In addition, the catechins did not affect the phosphorylation status of the insulin signal pathway. Thus, catechin suppressed adipocyte differentiation accompanied by the down-regulation of PPARγ2, C/EBPα, and GLUT4. These results suggest that tea catechin prevents obesity through the suppression of adipocyte differentiation.

• 社団法人 日本農芸化学会の論文
• 2004-11-23

著者

• HASHIMOTO Takashi

  Department of Biochemistry, Shinshu University School of Medicine

• ASHIDA Hitoshi

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

• Kanazawa K

  Laboratory Of Food And Nutritional Chemistry Graduate School Of Agriculture Kobe University

• Kanazawa Kazuki

  Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.

• Kanazawa Kazuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• FURUYASHIKI Takashi

  Department of Biofunctional Chemistry, Graduate School of Science and Technology, Kobe University

• NAGAYASU Hironobu

  Department of Biofunctional Chemistry, Graduate School of Science and Technology, Kobe University

• AOKI Yukiko

  Department of Biofunctional Chemistry, Graduate School of Science and Technology, Kobe University

• BESSHO Hiroaki

  Department of Biofunctional Chemistry, Graduate School of Science and Technology, Kobe University

• Kanazawa K

  Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.

• Kanazawa Kazuki

  Department Of Biosystems Science Graduate School Of Agricultural Science Kobe University

• Ashida H

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

• Ashida Hitoshi

  Department Of Utilization Of Biological Resources Kobe University

• Ashida Hitoshi

  Department Of Agricultural Chemistry Kobe University

• Ashida Hitoshi

  Graduate School Of Science & Technology Division Of Life Science Kobe University

• Hashimoto T

  Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.

• Ashida Hitoshi

  Department Of Life Science Graduate School Of Science And Technology Kobe University

• Ashida Hitoshi

  Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University:research Center

• Ashida Hitoshi

  Res. Center For Food Safety And Security Graduate School Of Agricultural Sci. Kobe Univ.

• Aoki Yukiko

  Department Of Biofunctional Chemistry Graduate School Of Science And Technology Kobe University

• Bessho Hiroaki

  Department Of Biofunctional Chemistry Graduate School Of Science And Technology Kobe University

• Hashimoto T

  Graduate School Of Agricultural Sci. Kobe Univ.

• Nagayasu Hironobu

  Department Of Biofunctional Chemistry Graduate School Of Science And Technology Kobe University

• Furuyashiki Takashi

  Department Of Biofunctional Chemistry Graduate School Of Science And Technology Kobe University

• Hashimoto Takashi

  Department Of Biochemistry Shinshu University School Of Medicine

• Hashimoto Takashi

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

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