The Expression Profile of Lectin Differs from That of Seed Storage Proteins in Castanea crenata Trees
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概要
- 論文の詳細を見る
Using Northern blot analysis, the expression of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) agglutinin (CCA) gene was compared with that of its seed storage protein (SSP) gene. After cDNA cloning of SSP, the expression profile of SSP mRNA and CCA mRNA were compared. SSP mRNA was seed-specific, while CCA mRNA was expressed in the stems and flowers (both male and female) as well as in the seeds. Whereas extracts from all organs observed using Western blot analysis exhibited positive signals, in seeds, large expressions of SSP mRNA were restricted to the late maturation and harvest stages. Levels were maintained during the dormant period. No expression was observed during the germination stage. In contrast, CCA mRNA expression was maintained at a high level during development, was at a relatively low level during dormancy, and showed subsequent high expression during germination. These results suggest that one of the physiological roles of CCA is to act as a vegetative storage protein. But since protein expression did not coincide with that of mRNA, the expression of CCA may be regulated both at the transcription and the translation levels.
- 社団法人 日本農芸化学会の論文
- 2004-08-23
著者
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Yagi Fumio
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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Nomura Kohji
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
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Mizuno Masashi
Graduate School Of Science And Technology Kobe University
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Nakamura Sachiko
Graduate School Of Science And Technology Kobe University
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Yagi Fumio
Department Of Behavioral Neuroscience Kochi Medical School
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Nomura K
Kushimoto Marine Park Center Wakayama Jpn
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Nomura Keiichi
Faculty Of Agriculture Kobe University
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Ikegami Ayako
Faculty Of Agriculture Kobe University
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Yagi Fumio
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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YAGI Fumio
Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University
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Mizuno Masashi
Graduate School of Agricultural Science, Kobe University
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