Differential Expression of Two Cathepsin Es during Metamorphosis-Associated Remodeling of the Larval to Adult Type Epithelium in Xenopus Stomach
スポンサーリンク
概要
- 論文の詳細を見る
Cathepsin E (CE) was purified from the foregut of Xenopus laevis tadpoles as a mature dimeric form. The purified enzyme was a typical CE among aspartic proteinases with respect to pH dependence of proteolytic activity, susceptibility to pepstatin, and having N-linked high-mannose type oligosaccharide chains. We isolated two cDNAs for the CE (CE 1 and CE 2) from adult stomach. The amino acid sequence of the N-terminal region of the purified CE coincided with the corresponding sequence predicted from CE 1. Northern blot analysis and in situ hybridization were performed. The CE 1 mRNA was highly expressed in surface mucous cells and gland cells constituting the larval epithelium of the foregut of pro-metamorphic tadpoles. As metamorphosis began and progressed, CE 1 mRNA drastically decreased in amount, and subsequently both CE 1 and CE 2 mRNAs gradually increased. The increase in CE 2 mRNA was detected shortly after the increase in CE 1 mRNA. The decrease in CE 1 expression correlated with degeneration of the larval type epithelium, while the increases in both CE 1 and CE 2 expression correlated with formation of the adult type epithelium. Thus, cathepsin E gene expression was differentially regulated during metamorphosis-associated remodeling of the larval to adult type epithelium in stomach.
- 社団法人 日本生化学会の論文
著者
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Nomura Kohji
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
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Iuchi Ichiro
Life Science Institute Sophia University
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KOBAYASHI Ken-ichiro
Life Science Institute, Sophia University
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Yasumasu Shigeki
Life Sci. Inst. Sophia Univ.
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Kobayashi Ken-ichiro
Life Science Institute Sophia University
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Ikuzawa Masayuki
Life Science Institute Sophia University
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Inokuchi Tomofumi
Department Of Biology Faculty Of Education Utsunomiya University
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