Isolation of the rpoD Gene Encoding the Principal Sigma Factor of the Deep-sea Piezophilic Bacterium Shewanella violacea Strain DSS12 and Its Overexpression in Escherichia coli
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概要
- 論文の詳細を見る
The gene encoding the principal σ factor (rpoD) of the piezophilic bacterium Shewanella violacea was cloned and sequenced. The rpoD gene was found to encode a polypeptide consisting of 614 amino acid residues, showing 75.6 and 64.3% identity to those of Escherichia coli and Pseudomonas putida, respectively. Comparison with E. coli σ^<70> and P. putida σ^<70> showed that significant similarity exists in four conserved regions known to be required for promoter recognition and core binding. Using an expression plasmid harboring the rpoD gene, the S. violacea σ^<70> factor was overexpressed in E. coli and successfully purified to near homogeneity.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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Kawano H
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Nakasone K
School Of Engineering Kinki Univ.
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Kato C
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Kato C
Extremobiosphere Research Center Jamstec
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KATO Chiaki
The DEEPSTAR Group, Japan Marine Science and Technology Center(JAMSTEC)
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Horikoshi Koki
The DEEP STAR Group, Japan Marine Science and Technology Center
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IKEGAMI Akihiko
Department of Oral Biology, State University of New York
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NAKASONE Kaoru
The DEEPSTAR Group, Japan Marine Science and Technology Center
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IKEGAMI Akihiko
The DEEP STAR Group, Japan Marine Science and Technology Center
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Ikegami Akihiko
Department Of Oral Biology State University Of New York
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Horikoshi K
Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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Nakasone Kaoru
School Of Engineering Kinki Univ.
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Ikegami Ayako
Faculty Of Agriculture Kobe University
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Kato Chiaki
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Kato Chiaki
Marine Biodiversity Research Program Japan Marine Science And Technology Center (jamstec)
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Horikoshi Koki
The Deep Star Group Japan Marine Science And Technology Center
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Kato Chiaki
The Deep Star Group Japan Marine Science And Technology Center
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