Preparation of Antibody against Antitumor β-Glucan in Grifola frondosa and Its Application

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概要

• 論文の詳細を見る

• Antibodies against an antitumor β-glucan purified from Grifola frondosa (GGF) were raised in the rabbit by subcutaneous immunization. Our antibodies reacted significantly with GGF by an ELISA inhibition assay. The antibodies did not recognize other polysaccharides such as laminarin and pustulan, but reacted somewhat with lentinan, whose structure is similar to GGF. It was demonstrated that GGF could be measured by ELISA using antibodies. In addition, the effects of the storage temperature on GGF content during storage were measured using our antibody. GGF content was 24.7 μg/g fresh weight (f.w.) at zero time storage, and little change occurred during storage of the mushroom for 7 days at 5℃. However, a drastic decrease to 11.4 μg/g f.w. occurred after 7 days of storage at 20℃. These results suggest that storage at low temperatures is desirable to maintain the quality of GGF.

• 社団法人　日本食品科学工学会の論文
• 1999-11-01

著者

• MINATO Ken-ichiro

  Miyagi Agricultural College

• Mizuno Masashi

  Division Of Science Of Biological Resources The Graduate School Of Science And Technology

• Mizuno Masashi

  Graduate School Of Science And Technology Kobe University

• Mizuno Masashi

  Nagano Japan Radio Co. Ltd. Currently With Kyushu Institute Of Technology

• Mizuno M

  Kobe Univ. Kobe Jpn

• Terai H

  Kobe Univ. Kobe

• Minato Kenichiro

  Miyagi Agricultural College

• Mizuno M

  Pharmaceutical Research Center Mochida Pharmaceutical Co. Ltd.

• Morimoto M

  Graduate School Of Science And Technology Kobe University

• MINATO Ken-ichiro

  Graduate School of Science and Technology, Kobe University

• KAWAKAMI Sachiko

  Graduate School of Science and Technology, Kobe University

• TATSUOKA Shigenobu

  Graduate School of Science and Technology, Kobe University

• TSUCHIDA Hironobu

  Graduate School of Science and Technology, Kobe University

• YAMAKAWA Akio

  Graduate School of Science and Technology, Kobe University

• TERAI Hirofumi

  Graduate School of Science and Technology, Kobe University

• Kawakami Sachiko

  Division Of Life Science Graduate School Of Science And Technology Kobe University:tottori Mycologic

• Minato Ken-ichiro

  School Of Food Agricultural And Environmental Sci. Miyagi Univ.

• Tsuchida Hironobu

  Graduate School Of Science And Technology Kobe University

• Tatsuoka Shigenobu

  Graduate School Of Science And Technology Kobe University

• Yamakawa Akio

  Graduate School Of Science And Technology Kobe University

• YAMAKAWA Akio

  Graduate School of Environmental and Life Science, Okayama University

• Mizuno Masashi

  Graduate School of Agricultural Science, Kobe University

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