Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains
スポンサーリンク
概要
- 論文の詳細を見る
Cycad leaf lectin (CRLL), a mannose-recognizing jacalin-related lectin (mJRL), was first cloned as a gymnosperm lectin and expressed. The cDNA sequence of CRLL (DDBJ, accession no. AB198328), coding 291 amino acid residues, has a tandem repeat of about 150 amino acids divided into N- and C-terminal domains as Japanese chestnut mJRL. Sequence alignment showed deletion and insertion of the sequence, and its putative carbohydrate-binding sites showed some differences from other JRLs. PCR analysis showed that this lectin was expressed in the cycad leaf but not in the root or seed. Recombinant CRLL (rCRLL) was expressed in Escherichia coli and purified by affinity chromatography after refolding procedures. Properties of active rCRLL appeared to be almost the same as those of native CRLL.
- 社団法人 日本農芸化学会の論文
- 2007-09-23
著者
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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Fukuda Ryouichi
Department of Biotechnology, The University of Tokyo
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Deng Lan
Department of Biotechnology, The University of Tokyo
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KAKIHARA Toru
Department of Biotechnology, The University of Tokyo
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Yagi Fumio
Applied Biological Chemistry The United Graduate School Of Agricultural Sciences Kagoshima Universit
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Nomura Keiichi
Faculty Of Agriculture Kobe University
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Haraguchi Tomokazu
Department Of Environment Science Saga University
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Haraguchi Tomokazu
Applied Biological Chemistry The United Graduate School Of Agricultural Sciences Kagoshima Universit
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Kakihara Toru
Department Of Biotechnology The University Of Tokyo
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Fukuda Ryouichi
Department Of Biotechnology The University Of Tokyo
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Deng Lan
Department Of Biotechnology The University Of Tokyo
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Nomura Keiichi
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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YAGI Fumio
Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University
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