Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• In this study, we addressed the question of which genes are transcriptionally co-regulated upon proliferation of the endoplasmic reticulum, as induced by an experimental overexpression of cytochrome P450Cm2 (CYP52A4) in Saccharomyces cerevisiae. Using the mRNA differential display technique, six genes were found to be up-regulated : ASN2,MDJ1,YLR194c, YNL208w, YER175,and YGL121c. Genes coding for Dur1.2p, Dal2p, and Sps19p were down-regulated. Two strongly induced genes, which were found to accommodate the peroxisome box (YLR194c) and a 10-bp consensus sequence of genes involved in lipid metabolism (YNL208w) in their promoter regions, were further analyzed with respect to the course of induction, the necessity of the P450 membrane anchor for induction, and the effects of gene disruption on P450Cm2 overexpression. We found that both genes are not essential to overproduce P450Cm2,but their induction was dependent on P450Cm2 membrane integration.

• 社団法人日本農芸化学会の論文
• 2000-09-23

著者

• Ohta Atsutane

  Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.

• Takagi M

  Department Of Biotechnology The University Of Tokyo

• Ohta Akinori

  Department of Agricultural Chemistry, The University of Tokyo

• Takagi Masamichi

  Department of Agricultural Chemistry, The University of Tokyo

• Ohta A

  Dep. Of Biotechnology The Univ. Of Tokyo

• Ohta A

  Univ. Tokyo Tokyo

• Ohta Akinori

  Department Of Agricultural Chemistry The University Of Tokyo

• Ogura Atsushi

  Department Of Biotechnology The University Of Tokyo

• Takahashi Maki

  Department Of Biotechnology The University Of Tokyo

• Takagi Masamichi

  Department Of Biotechnology University Of Tokyo

• Takagi Masamichi

  Department Of Biotechnology The University Of Tokyo

• Takagi Masamichi

  Department Of Agricultural Chemistry The University Of Tokyo

• Takagi M

  Department Of Biotechnology University Of Tokyo

• Ogura A

  Meiji University

• ZIMMER Thomas

  Department of Biotechnology, The University of Tokyo

• TAKEWAKA Tsuyoshi

  Department of Biotechnology, The University of Tokyo

• ZIMMER RoseMarie

  Department of Biotechnology, The University of Tokyo

• Zimmer Thomas

  Department Of Biotechnology The University Of Tokyo:friedrichschiller University Jena Institute Of P

• Zimmer Rosemarie

  Department Of Biotechnology The University Of Tokyo:friedrichschiller University Jena Institute Of P

• Takahi Masamichi

  Department Of Biotechnology The University Of Tokyo

• Takewaka Tsuyoshi

  Department Of Biotechnology The University Of Tokyo

• Zimmer Thomas

  Department of Biotechnology The University of Tokyo

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