Stabilization by the Mini-F Fragment of a pBR322 Derivative Bearing the Tryptophan Operon in Escherichia coli
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概要
- 論文の詳細を見る
In an Escherichia coli K-12 strain (trpA trpE tna) cultured in LB broth without selective pressure, a pBR322 derivative bearing the E. coli tryptophan operon (pBR322-trp) was rapidly lost: after 27 cell-number doublings, only 7% cells retained both tryptophan prototrophy (Trp+) and ampicillin resistance (Apr), and 17% were Apr but Trp-. Insertion of the mini-F DNA from F factor into this plasmid effectively suppressed both the plasmid loss and the discoordinate loss of Trp+: the percentage of Trp- cells per cell-number doubling was decreased more than 100-fold. Partial derepression of the trp operon due to 3-indole acrylic acid further decreased the stability of the pBR322-trp but not that of the mini-F-inserted pBR322-trp.
- 社団法人 日本農芸化学会の論文
著者
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Shibuya Isao
Department Of Dental Biomaterials Nihon University School Of Dentistry At Matsudo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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YUKAWA Hideaki
Central Research Laboratory, Mitsubishi Petrochemical Co., Ltd.
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KURUSU Yasurou
Central Research Laboratory, Mitsubishi Petrochemical Co., Ltd.
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SHIMAZU Mitsunobu
Central Research Laboratory, Mitsubishi Petrochemical Co., Ltd.
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TERASAWA Masato
Central Research Laboratory, Mitsubishi Petrochemical Co., Ltd.
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OHTA Akinori
Department of Biochemistry, Saitama University
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SHIBUYA Isao
Department of Biochemistry, Saitama University
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