Preparation of Protoplasts of Rhizopus niveus and Their Transformation with Plasmid DNA(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
An efficient method for protoplast formation of Rhizopus niveus, a filamentous fungus with high capability to secrete enzymes out of the cells, was established using three lytic enzymes in combination, and a plasmid with a selection marker for transformation was constructed by transcriptional fusion of a Rhizopus promoter with the G418 resistance gene derived from Tn903. Then the protoplasts were mixed with the plasmid DNA in the presence of polyethylene glycol 4000 and CaCl_2. G418-resistant colonies were obtained, indicating that transformation was successful. Southern blot analysis of DNA from a transformant shows that the introduced DNA was present not only integrated into the host chromosome but also replicating extrachromosomally, and that it was rearranged with host DNA during the course of cell cultivation. This is the first report of a transformation system for Rhizopus. This system will open the possibility for breeding R. niveus at the molecular level.
- 社団法人日本農芸化学会の論文
- 1990-10-23
著者
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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YANAI Koji
Department of Biotechnology, The University of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Yanai Koji
Department Of Biotechnology The University Of Tokyo:bio Science Laboratories Meiji Seika Kaisha Ltd.
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YANAI Koji
Department of Agricultural Chemistry, The University of Tokyo
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