Construction of Chicken-Mouse Chimeric Antibody and Immunogenicity in Mice
スポンサーリンク
概要
- 論文の詳細を見る
Chicken monoclonal antibodies are potentially useful for diagnostic research and have clinical applications, as chicken show higher potential for antibody production with mammalian-conserved biological molecules. However, the applications of chicken antibodies are limited because of their immunogenicity in mammals. To overcome this problem, we have constructed a chicken-mouse chimeric antibody containing the chicken variable region and the mouse constant region. This chimeric antibody retained similar binding affinities as the parental chicken antibody. The chimeric antibody was also producible as an ascitic antibody in BALB/c mice. Furthermore, when the chimeric antibody was administered to mice, it did not provoke the mouse anti-chicken antibody response. These results indicate that the chimeric antibody is suitable for application to preclinical mouse studies.
- 公益社団法人 日本獣医学会の論文
著者
-
MATSUDA Haruo
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Bios
-
FURUSAWA Shuichi
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Bios
-
Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
-
MATSUDA Haruo
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University
-
FURUSAWA Shuichi
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University
-
TATEISHI Yoshiko
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University
-
NISHIMICHI Norihisa
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University
関連論文
- Induction of Immune Suppression in the Chick by an Optimal Dose of an Immunizing Antigen in Presence of Its Specific Maternal Antibody
- Prolonged Suppression of Chick Humoral Immune Response by Antigen Specific Maternal Antibody
- R-Dependency of the Hardness Of Perovskite-Type RRh_3B Compounds (R = La, Gd, Lu and Sc) : Structure and Mechanical and Thermal Properties of Condensed Matter
- Solid Solution Range of Boron, Microhardness and Magnetic Properties of the Perovskite-Type GdRh_3B Obtained by Arc-melting Synthesis
- Production of Recombinant Der fI (a major mite allergen) by Aspergillus oryzae
- Production of Recombinant Der fI with the Native IgE-Binding Activity Using a Baculovirus Expression System
- Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
- Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
- Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
- Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
- Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
- Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
- Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
- Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
- Transformation of a Leu^- Mutant of Rhizopus niveus with the leuA Gene of Mucor circinelloides
- Preparation of Protoplasts of Rhizopus niveus and Their Transformation with Plasmid DNA(Microbiology & Fermentation Industry)
- High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
- The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
- A Protein Kinase C-Encoding Gene, pkcA, Is Essential to the Viability of the Filamentous Fungus Aspergillus nidulans
- Construction of Chicken-Mouse Chimeric Antibody and Immunogenicity in Mice