Construction of a Genetic Transformation System for a Freeze-tolerant Yeast Kluyveromyces thermotolerans

スポンサーリンク

概要

• 論文の詳細を見る

• We have developed a genetic transformation system for a freeze-tolerant yeast Kluyveromyces thermotolerans. K. thermotolerans spheroplasts could be transformed with a YPp-type vector containing an autonomously replicating (ARS) of Saccharomyces cerevisiae. However, transformation with a Yep-type vector containing a replication origin of S. cerevisiae 2μM DNA was not successful. The cycloheximide resistance gene (RIM-C) of Candida maltosa and the URA3 gene of S. cerevisiae were successfully used to transform a prototrophic strain of K. thermotolerans and an Ura-mutant of this yeast isolated in this study, respectively. Transformation was also possible by using intact cells treated with lithiukm salts or thiol compounds. The YRp-type vectors were maintained as plasmids in the transformants under selective conditions. This is the first report of successful transformation of K. thermotolerans.

• 社団法人日本農芸化学会の論文
• 1992-02-23

著者

• HINO Akihiro

  National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

• Murao S

  Department Of Agricultural Chemistry The University Of Tokyo

• Takagi Masamichi

  Department of Agricultural Chemistry, The University of Tokyo

• Kawai S

  Yamaguchi Univ. School Of Medicine Ube Jpn

• Kawai Shinya

  Department Of Orthopedic Surgery Yamaguchi University School Of Medicine

• YANO KEIJI

  Department of BioEngineering, Nagaoka University of Technology

• TAKANO Hiroyuki

  National Food Research Institute, Tsukuba Science City

• Yano K

  Dept. Bioeng. Nagaoka Univ. Tech.:jrdc

• Yano Keiji

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Takahashi Maki

  Department Of Biotechnology The University Of Tokyo

• WONGKHALAUNG Chakamas

  Institute of Food Research and Product Development, Kasetsart University

• MURAO Satoru

  Department of Agricultural Chemistry, The University of Tokyo

• Kawai Shinya

  Graduate School Of Engineering Tokyo University Of Agriculture And Technology

• Kawai Shinya

  Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied

• Takagi Masamichi

  Department Of Biotechnology University Of Tokyo

• Takagi Masamichi

  Department Of Biotechnology The University Of Tokyo

• Takagi Masamichi

  Department Of Agricultural Chemistry The University Of Tokyo

• Takano Hiroyuki

  Agricultural Chemicals Research Laboratory Sumitomo Chemical Co. Ltd.

• Takano Hiroyuki

  National Food Research Institute Ministry Of Agriculture And Forestry

• Takagi M

  Department Of Biotechnology University Of Tokyo

• Murao Satoru

  Department Of Agricultural Chemistry The University Of Tokyo

• Hino Akihiro

  National Food Research Institute Ministry Of Agriculture Forestry And Fisheries

• Wongkhalaung C

  Institute Of Food Research And Product Development Kasetsart University

• TAKANO Hiroyuki

  National Food Research Institute

• HINO Akihiro

  National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO)

関連論文

• Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
• Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
• Multiplex PCR法を用いた組換えトウモロコシ5系統からの組換え遺伝子の検知法
• Duplex PCR法を用いた遺伝子組換えパパイヤ改良検知法
• 組織化学的手法を用いた遺伝子組換えパパイヤ(55-1)検知法の確立
• Evidence That the Expression of the Gene for NADPH-Cytochrome P-450 Reductase is n-Alkane-inducible in Candida maltosa
• Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
• Isolation and Characterization of ECT1 Gene Gncoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae^1
• Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
• Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
• Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
• Rapid Quantification Methods for Genetically Modified Maize Contents Using Genomic DNAs Pretreated by Sonication and Restriction Endonuclease Digestion for a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard
• A Detection Method of CryIAc Protein for Identifying Genetically Modified Rice Using the Lateral Flow Strip Assay
• Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard
• New Qualitative Detection Methods of Genetically Modified Potatoes(Highlighted paper selected by Editor-in-chief)(Analytical Biochemistry)
• Purification and Properties of a Galacturonic Acid-releasing Exopolygalacturonase from a Strain of Bacillus
• Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
• Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
• Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
• Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
• Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
• Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
• Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
• Hyperproduction of Sebaceous cis-6-Hexadecenoic Acid by Esterase-Reduced Mutant of Rhodococcus sp. Strain(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Detergent Alkaline Proteases : Enzymatic Properties, Genes, and Crystal Structures
• Deduced Amino Acid Sequence and Possible Catalytic Residues of a Thermostable, Alkaline Cellulase from an Alkaliphilic Bacillus Strain
• Thermostabilization by Proline Substitution in an Alkaline, Liquefying α-Amylase from Bacillus sp. Strain KSM-1378
• Amino Acid Sequence and Stereoselective Hydrolytic Reaction of an Endo-1, 4-β-glucanase from a Bacillus Strain
• Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis
• Construction, Purification, and Properties of a Truncated Alkaline Endoglucanase from Bacillus sp. KSM-635
• Molecular Cloning and Nucleotide Sequence of the Gene for an Alkaline Protease from the Alkalophilic Bacillus sp. KSM-K16
• Alkali-Resistant, Alkaline Endo-1,4-β-glucanase Produced by Bacillus sp. PKM-5430
• Pseudomonas fluorescens KKL101, a Benzoic Acid Degrader in a Mixed Culture That Degrades Biphenyl and Polychlorinated Biphenyls
• The Wound-Healing Effect of Fibrin Glue for Tracheal Anastomosis in Experimental Pulmonary Surgery
• Reinforcing Effect of the New Synthetic Bioabsorbable Sealant (ADVASEAL^) for Bronchial Stumps after Pulmonary Resections in Pigs
• Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
• Construction of a Genetic Transformation System for a Freeze-tolerant Yeast Kluyveromyces thermotolerans
• Isolation and Sequencing of a Gene, C-ADE1, and Its Use for a Host-Vector System in Candida maltosa with Two Genetic Markers(Microbiology & Fermentation Industry)
• Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
• Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
• Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
• Expression of Plant Group 2 and Group 3 lea Genes in Saccharomyces cerevisiae Revealed Functional Divergence among LEA Proteins
• Video-Assisted Thoracic Surgery for Patients with Lung Cancer and Interstitial Pneumonia
• Preoperative Evaluation of a Tracheal Bronchus by Three-Dimensional 64-Row Multidetector-Row Computed Tomography (MDCT) Bronchography and Angiography : Report of a Case
• The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
• Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
• Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
• Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
• Radical-capturing Reaction of 5,7,3', 4'-Tetramethylquercetin with the AIBN Radical Initiator
• Antioxidative activity of tree phenolic constituents I : Radical-capturing reaction of flavon-3-ols with radical initiator
• Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
• Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
• Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
• Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
• Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
• Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
• Transformation of a Leu^- Mutant of Rhizopus niveus with the leuA Gene of Mucor circinelloides
• Preparation of Protoplasts of Rhizopus niveus and Their Transformation with Plasmid DNA(Microbiology & Fermentation Industry)
• Isolation and Some Properties of Acid Phosphatase-1^1 from Tomato Leaves(Biological Chemistry)
• High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
• Isolation of a Genomic Clone Encoding RNase Rh of Rhizopus niveus and Characterization of Its 5-Terminal Region
• Unexpected Phase Behaviors of Poly(fumarate)s Carrying Tolane-based Mesogenic Side Chains
• Control of Phase-transition Behavior and Polymerization Capability by Methyl Addition to Dialkyl Fumarates Bearing Cyanobiphenyl Mesogens
• Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
• A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
• Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
• Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
• Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
• Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
• Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
• Molecular-Dynamics Study of Aqueous Solution of Trehalose and Maltose: Implication for the Biological Function of Trehalose
• Effects of Trehalose on the Water Structure in Yeast Cells as Studied by in vivo ^1HNMR Spectroscopy
• Specific Accumulation of Polysaccharide-Linked Hydroxycinnamoyl Esters in the Cell Walls of Irregularly Shaped and Collapsed Internode Parenchyma Cells of the Dwarf Rice Mutant Fukei 71
• EN-P35 Fluorometric assay substrate for dioxin degrading microorganisms-synthesis of dioxin-analogous fluorometric assay substrate(Section VI Environmental Biotechnology)
• EN-P29 Functional analysis of dioxin-degrading microorganism using the fluorometric substrate and chlorinated dioxin(Section VI Environmental Biotechnology)
• Separation of Chromosomal DNA Molecules of Candida maltosa on Agarose Gels Using the OFAGE Technique(Microbiology & Fermentation Industry)
• Hybrid aspen with a transgene for fungal manganese peroxidase is a potential contributor to phytoremediation of the environment contaminated with bisphenol A
• Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen
• Molecular cloning of the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene that contributes to the construction of a new transformation system in Coriolus versicolor
• Isolation and Analysis of Cinnamic Acid 4-Hydroxylase Homologous Genes from a Hybrid Aspen, Populus kitakamiensis
• REGULATION AND EXPRESSION OF PHENYLALANINE AMMONIA-LYASE FROM A WOODY PLANT
• ANALYSIS OF CINNAMIC ACID 4-HYDROXYLASE GENES FROM A WOODY PLANT(Populus kitakamiensis)
• Degradation and Solubilization of ^C-, ^C-side Chain Labeled Synthetic Lignin (Dehydrogenative Polymerizate) by Laccase III of Coriolus versicolor
• Nucleotide Sequence for the Genomic DNA Encoding an Anionic Peroxidase Gene from a Hybrid Poplar, Populus kitakamiensis
• Oxazolomycin Esters, Specific Inhibitors of Plant Transformation
• Nucleotide Sequence of the Gene for an Alkaline Endoglucanase from an Alkalophilic Bacillus and Its Expression in Escherichia coli and Bacillus subtilis
• Nucleotide Sequence of the Gene That Encodes a Neopullulanase from an Alkalophilic Bacillus
• Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
• Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyces cerevisiae
• LC/MSによる5-Enolpyruvylshikimate-3-phosphate Synthaseの酵素活性の測定
• トウモロコシからの組換え遺伝子の検知法
• Expression of leptin receptor (Ob-R) isoforms and signal transducers and activators of transcription (STATs) mRNAs in the mouse taste buds
• Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
• Self-cloning of Genes for n-Alkane Assimilation from Candida maltosa(Microbiology & Fermentation Industry)
• Yeast Flocculation Mediated by Over-expression of the Chitin Synthase 2 Gene in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
• Purification of Cytochrome P-450alk from n-Alkane-grown Cells of Candida maltosa, and Cloning and Nucleotide Sequencing of the Encoding Gene(Microbiology & Fermentation Industry)
• Evidence That More Than One Gene Encodes n-Alkane-inducible Cytochrome P-450s in Candida maltosa, Found by Two-Step Gene Disruption(Microbiology & Fermentation Industry)
• Evaluation of Quantitative PCR Methods for Genetically Modified Maize (MON863, NK603, TC1507 and T25)
• Conditions for Isolation of and Colony Formation by Mycelial Protoplasts of Coprinus macrorhizus
• Subcloniag and Nucleotide Sequencing of an ARS Site of Candida maltosa Which Also Functions in Saccharomyces cerevisiae

もっと見る 閉じる

スポンサーリンク