Thermostabilization by Proline Substitution in an Alkaline, Liquefying α-Amylase from Bacillus sp. Strain KSM-1378
スポンサーリンク
概要
- 論文の詳細を見る
α-Amylase (LAMY) from alkaliphilic Bacillus sp. strain KSM-1378 is a novel semi-alkaline enzyme which has 5-fold higher specific activity than that of a Bacillus licheniformis enzyme. The Arg124 in LAMY was replaced with proline by site-directed mutagenesis to increase thermostability of the enzyme. The wild-type and engineered LAMYs were very similar with respect to specific activity, kinetic values, pH-activity curve, and degree of inhibition by chelating reagents. Thermostability and structure stiffness of LAMYs as measured by fluorescence were increased by the proline substitution. The change of Arg124 to proline is assumed to stabilize the loop region involving aminio acid residues from 122 to 134. This is the first report that thermostability of an α-amylase is improved by proline substitution.
- 社団法人日本農芸化学会の論文
- 1999-09-23
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Hatada Yuji
Tochigi Research Laboratories Of Kao Corporation
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Ozawa T
Univ. Tsukuba Ibaraki Jpn
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Hayashi Y
Research Institute For Food Science Kyoto University
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Hagihara H
Tochigi Research Laboratories Kao Corporation
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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Kobayashi Shunsuke
Department Of Electronic Engineering (div. Of Engineering Sciences) Faculty Of Technology Tokyo Univ
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Araki Hiroyuki
Tochigi Research Laboratories, Kao Corporation
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Hagihara Hiroshi
Tochigi Research Laboratories, Kao Corporation
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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IGARASHI Kazuaki
Tochigi Research Laboratories of Kao Corporation
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IKAWA-KITAYAMA Kaori
Tochigi Research Laboratories of Kao Corporation
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HAKAMADA Yoshihiro
Tochigi Research Laboratories of Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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OZAWA Tadahiro
Tochigi Research Laboratories of Kao Corporation
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HAYASHI Yasuhiro
Tochigi Research Laboratories of Kao Corporation
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ENDO Keiji
Tochigi Research Laboratories of Kao Corporation
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IKAWA Kaori
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Igarashi Kaori
Graduate School Of Human Life Sciences Showa Women's University
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Ozawa T
Tochigi Res. Lab. Kao Corp. Tochigi Jpn
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Igarashi Kazuei
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Kobayashi Shunsuke
Science University Of Tokyo
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Igarashi K
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ito S
National Industrial Research Institute
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Hayashi Y
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science Kyoto Pharmaceut
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Araki Hiroyuki
Tochigi Research Laboratories Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Araki H
Tochigi Research Laboratories Kao Corporation
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Kobayashi Shun-ichi
Department Of Physics School Of Science University Of Tokyo
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Igarashi Kazuhiko
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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Kobayashi Shunichi
Department Of Physics Graduate School Of Science The University Of Tokyo
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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Hagihara Hiroshi
Tochigi Research Laboratories Kao Corporation
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KOBAYASHI Shun-ichi
Department of Physics, University of Tokyo
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Kobayashi Shunichi
Department of Physics, Faculty of Science, University of Tokyo
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