An Alkaline Amylopullulanase from Alkalophilic Bacillus sp. KSM-1378; Kinetic Evidence for Two Independent Active Sites for the α-1,4 and α-1,6 Hydrolytic Reactions
スポンサーリンク
概要
- 論文の詳細を見る
Alkalophilic Bacillus sp. KSM-1378 produces an alkaline amylopullulanase that hydrolyzes both α-1, 4 linkages in amylose, amylopectin, and glycogen and α-1, 6 linkages in pullulan. The hydrolytic activities against amylose and pullulan were specifically inhibited by maltotriose (K_i=0. 5mM), isomaltitol (K_i=5. 2mM), and methyl α-D-galactoside (K_i=40mM) and by β-cyclodextrin (K_i=0. 9mM), α-cyclodextrin (K_i=11mM), and raffinose (K_i=31mM), respectively, in a competitive manner in each case. Inhibition by N-bromosuccinimide of the α-amylase activity was prevented by amylose but not by pullulan, while inhibition by N-bromosuccinimide of the pullulanase activity was prevented by pullulan but not by amylose. Kinetics of reactions in the simultaneous presence of amylose and pullulan indicated that the observed rates of formation of products closely matched those predicted by a kientic model in which the α-1, 4 and α-1, 6 hydrolytic reactions were catalyzed at two independent active sites. Incubation of the enzyme at 40℃ and pH 9. 0 caused complete inactivation of the amylase activity within 4 days, but the pullulanase activity remained at the original level under the same conditions. This alkaline amylopullulanase can, therefore, be considered to be a "two-headed" enzyme molecule.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
-
Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
-
ITO Susumu
Tochigi Research laboratories, Kao Corporation
-
Saeki Katsuhisa
Tochigi Research Laboratories of Kao Corporation
-
IGARASHI Kazuaki
Tochigi Research Laboratories of Kao Corporation
-
IKAWA-KITAYAMA Kaori
Tochigi Research Laboratories of Kao Corporation
-
ARA Katsutoshi
Tochigi Research Laboratories of Kao Corporation
-
Kawai S
Tochigi Research Laboratories Kao Corporation
-
Igarashi Kaori
Graduate School Of Human Life Sciences Showa Women's University
-
Igarashi Kazuei
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
-
Igarashi K
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
-
Saeki K
Department Of Hematology Research Institute International Medical Center Of Japan
-
Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
-
Igarashi Kazuhiko
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
-
Ito Susumu
Tochigi Research Laboratories Kao Corporation
関連論文
- Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
- Effect of Various Radical Generators on Insulin-Dependent Regulation of Hepatic Gene Expression
- The Promoter of Mouse Transcription Repressor bach1 Is Regulated by Sp1 and Trans-Activated by Bach^1
- 2P007 大腸菌由来スペルミジンアセチルトランスフェラーゼのX線結晶構造解析(蛋白質-構造,第48回日本生物物理学会年会)
- Iron Absorption in Rats Increased by Yeast Glucan(Food & Nutrition Sience)
- Purification and Properties of a Galacturonic Acid-releasing Exopolygalacturonase from a Strain of Bacillus
- Novel Triene-β-lactone Antibiotics, Oxazolomycin Derivative and Its Isomer, Produced by Streptomyces sp. KSM-2690
- Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
- Hyperproduction of Sebaceous cis-6-Hexadecenoic Acid by Esterase-Reduced Mutant of Rhodococcus sp. Strain(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Detergent Alkaline Proteases : Enzymatic Properties, Genes, and Crystal Structures
- M_r 25 000 protein, substrate for protein serine/threonine kinases, is identified as a part of xenopus laevis vitellogenin B1
- 洗剤用α-アミラーゼのタンパク質工学
- A Serpin with M_r 43,000 Is a Binding Protein of M_r 25,000 Protein, a Substrate for Protein Ser/Thr Kinase Detected in Xenopus laevis Oocytes
- Deduced Amino Acid Sequence and Possible Catalytic Residues of a Thermostable, Alkaline Cellulase from an Alkaliphilic Bacillus Strain
- A New High-alkaline and High-molecular-weight Pectate Lyase from a Bacillus Isolate : Enzymatic Properties and Cloning of the Gene for the Enzyme
- Thermostabilization by Proline Substitution in an Alkaline, Liquefying α-Amylase from Bacillus sp. Strain KSM-1378
- Cloning and Sequencing of a High-alkaline Pectate Lyase Gene from an Alkaliphilic Bacillus Isolate
- Purification and Properties of a Low-molecular-weight, High-alkaline Pectate Lyase from an Alkaliphilic Strain of Bacillus
- Hyperexpression of the Gene for a Bacillus α-Amylase in Bacillus subtilis Cells : Enzymatic Properties and Crystallization of the Recombinant Enzyme
- Amino Acid Sequence and Stereoselective Hydrolytic Reaction of an Endo-1, 4-β-glucanase from a Bacillus Strain
- NADP-Specific Glutamate Dehydrogenase from Alkaliphilic Bacillus sp. KSM-635: Purification and Enzymatic Properties
- Separation of Functional Domains for the α-1,4 and α-1,6 Hydrolytic Activities of a Bacillus Amylopullulanase by Limited Proteolysis with Papain
- Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis
- Construction, Purification, and Properties of a Truncated Alkaline Endoglucanase from Bacillus sp. KSM-635
- An Alkaline Amylopullulanase from Alkalophilic Bacillus sp. KSM-1378; Kinetic Evidence for Two Independent Active Sites for the α-1,4 and α-1,6 Hydrolytic Reactions
- Molecular Cloning and Nucleotide Sequence of the Gene for an Alkaline Protease from the Alkalophilic Bacillus sp. KSM-K16
- Alkali-Resistant, Alkaline Endo-1,4-β-glucanase Produced by Bacillus sp. PKM-5430
- Decrease in Polyamines with Aging and Their Ingestion from Food and Drink
- Structural and Functional Relationship among Diamines in Terms of Inhibition of Cell Growth
- Polyamine Cytotoxicity in the Presence of Bovine SerumAmine Oxidase
- Identification of the Putrescine Recognition Site on PolyamineTransport Protein PotE
- Inhibition of Cell Growth by Combination of α-Difluromethylornithine and an Inhibitor of Spermine Synthase
- Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Saccharomyces cerevisiae Mutant Displaying β-Glucans on Cell Surface(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Characterization of Enzyme Responsible for N-Myristoylation of Octapeptide in Aqueous Solution without ATP and Coenzyme A from Pseudomonas aeruginosa(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 1F11-3 Characterization of the Enzyme Responsible for N-Myristoylation of Octapeptide in Aqueous Solution without ATP and CoA
- Relationship between Cell Morphology and Intracellular Potassium Concentration in Candida albicans
- Valinomycin Affects the Morphology of Candida albicans
- Deletion of MCD4 Involved in Glycosylphosphatidylinositol (GPI) Anchor Synthesis Leads to an Increase in β-1,6-Glucan Level and a Decrease in GPI-Anchored Protein and Mannan Levels in the Cell Wall of Saccharomyces cerevisiae(Microbial Physiology and Biot
- Analysis of Chitin at the Hyphal Tip of Candida albicans Using Calcofluor White
- Effects of a Solute Analogue p-Acetoacetanisidide on the Crystallization of p-Acetanisidide
- Carbohydrate-Active Enzymes from Alkaliphiles
- Radical-capturing Reaction of 5,7,3', 4'-Tetramethylquercetin with the AIBN Radical Initiator
- Antioxidative activity of tree phenolic constituents I : Radical-capturing reaction of flavon-3-ols with radical initiator
- Effect of Bacillus subtilis spo0A Mutation on Cell Wall Lytic Enzymes and Extracellular Proteases, and Prevention of Cell Lysis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Indispensable Glutamic Acid Residue-139 of NtpK Proteolipid in the Reaction of Vacuolar Na^+-Translocating ATPase in Enterococcus hirae
- Properties of the V_0 V_1 Na^+-ATPase from Enterococcus hirae and Its V_0 Moiety
- Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
- Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
- Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
- Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
- Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
- Deterioration of Superconductive Character of Y-Ba-Cu Oxides in Time Duration : Electrical Properties of Condensed Matter
- Electron Irradiation of Y-Ba-Cu Oxides : Electrical Properties of Condensed Matter
- Substrate Specificity of Regiospecific Desaturation of Aliphatic Compounds by a Mutant Rhodococcus Strain
- Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain
- Soy Protein Suppresses Gene Expression of Acetyl-CoA Carboxylase Alpha from Promoter PI in Rat Liver
- Developmental Transition of the Flavonoid Contents in Safflower Leaves during Stress-loaded Cultivation(Food & Nutrition Science)
- Reduction of Paraquat-induced Oxidative Stress in Rats by Dietary Soy Peptide(Food & Nutrition Science)
- Isolation of Enterococcus hirae Mutant Deficient in Low-affinity Potassium Uptake at Alkaline pH(Microbiology & Fermentation Technology)
- Polyphenol Increases in Safflower and Cucumber Seedlings Exposed to Strong Visible Light with Limited Water(Food & Nutrition Science)
- Preventive Effects of Dietary Cabbage Acylated Anthocyanins on Paraquat-induced Oxidative Stress in Rats
- Protective Effects of Dietary Nasunin on Paraquat-induced Oxidative Stress in Rats
- HISTOCHEMICAL DISTRIBUTION OF GUANASE IN HUMAN TISSUES WITH GUANINE IN BICINE BUFFER AS SUBSTRATE
- Histochemical distribution of guanase in various human tissues
- EN-P35 Fluorometric assay substrate for dioxin degrading microorganisms-synthesis of dioxin-analogous fluorometric assay substrate(Section VI Environmental Biotechnology)
- EN-P29 Functional analysis of dioxin-degrading microorganism using the fluorometric substrate and chlorinated dioxin(Section VI Environmental Biotechnology)
- Hybrid aspen with a transgene for fungal manganese peroxidase is a potential contributor to phytoremediation of the environment contaminated with bisphenol A
- Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen
- Isolation and Analysis of Cinnamic Acid 4-Hydroxylase Homologous Genes from a Hybrid Aspen, Populus kitakamiensis
- REGULATION AND EXPRESSION OF PHENYLALANINE AMMONIA-LYASE FROM A WOODY PLANT
- ANALYSIS OF CINNAMIC ACID 4-HYDROXYLASE GENES FROM A WOODY PLANT(Populus kitakamiensis)
- Degradation and Solubilization of ^C-, ^C-side Chain Labeled Synthetic Lignin (Dehydrogenative Polymerizate) by Laccase III of Coriolus versicolor
- Nucleotide Sequence for the Genomic DNA Encoding an Anionic Peroxidase Gene from a Hybrid Poplar, Populus kitakamiensis
- Oxazolomycin Esters, Specific Inhibitors of Plant Transformation
- Nucleotide Sequence of the Gene for an Alkaline Endoglucanase from an Alkalophilic Bacillus and Its Expression in Escherichia coli and Bacillus subtilis
- Nucleotide Sequence of the Gene That Encodes a Neopullulanase from an Alkalophilic Bacillus
- Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp.KSM-1876
- Alkaline Cellulases for Laundry Detergents : Production by Alkalophilic Strains of Bacillus and Some Properties of the Crude Enzymes(Microbiology & Fermentation Industry)
- Purification and Characterization of Multiple Carboxymethyl Cellulases from Bacillus sp. KSM-522(Microbiology & Fermentation Industry)
- Alkaline Cellulase for Laundry Detergents: Production by Bacillus sp. KSM-635 and Enzymatic Properties(Microbiology & Fermentation Industry)
- Neutrophilic Bacillus Strain, KSM-522, That Produces an Alkaline Carboxymethyl cellulase(Microbiology & Fermentation Industry)
- Comparative Composition of Brown Rice Lipids (Lipid Fractions) of Indica and Japonica Rices
- Enhanced Production of Extracellular Enzymes by Mutants of Bacillus That Have Acquired Resistance to Vancomycin and Ristocetin(Microbiology & Fermentation Industry)
- Occurrence of Unusual Fatty Acids in the Earthworm, Lumbricus terrestris (Food & Nutrition)
- Two-tone interaction in phase locking of cat cochlear nerve fibers
- Isofrequency planes in the brainstem auditory nuclei of the rat as revealed by the C-deoxyglucose method
- NapA Na^+/H^+ Antiporter as a Sodium Extrusion System Supplementary to the Vacuolar Na^+-ATPase in Enterococcus hirae
- Crystallization and Preliminary X-Ray Analysis of a Truncated Family A Alkaline Endoglucanase Isolated from Bacillus sp.
- High Level Expression of XMP Aminase in Escherichia coli and Its Application for the Industrial Production of 5'-Guanylic Acid
- Construction of a Plasmid for High Level Expression of XMP Aminase in Escherichia coli(Microbiology & Fermentation Industry)
- Inhibition of Glucose Catabolism by Aeration in Leuconostoc mesenteroides : Studies on Lactic Acid Bacteria (III).
- Chemical Composition of Lipids, Especially Triacylglycerol, in Grape Seeds(Food & Nutrition)
- Isolation and Functional Analysis of the Promoter Sequence of the Cry j 1 Gene, Which Encodes a Major Allergenic Protein in the Pollen of Japanese Cedar (Cryptomeria japonica)
- Effects of Dietary Nasunin on the Serum Cholesterol Level in Rats
- Effect of Various Radical Generators on Insulin-Dependent Regulation of Hepatic Gene Expression
- Regulation of the plasma cell transcription factor Blimp-1 gene by Bach2 and Bcl6
- Architecture and Dynamics of the Transcription Factor Network that Regulates B-to-Plasma Cell Differentiation
- Development of New Analytical System Using the Electron Cyclotron Resonance Ion Source and Heavy Ion Linear Accelerator
- Carbohydrate-Active Enzymes from Alkaliphiles