Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH_2(PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH_2(HIV-1p17^<gag>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.
- 社団法人日本生物工学会の論文
- 2008-03-25
著者
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Islam Nazneen
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma Masayuki
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Ooshima Hiroshi
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Ooshima H
Osaka City Univ. Osaka Jpn
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Ooshima Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ooshima Hiroshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Igarashi K
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Igarashi Koichi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Igarashi Koichi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Igarashi Koichi
Biology Research Laboratories:research And Development Division:tekada Chemical Industries Ltd.
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Tachibana Taro
Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City Unive
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Igarashi K
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma M
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma Masayuki
Second Department Of Oral And Maxillofacial Surgery Tokushima University School Of Dentistry
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Tachibana Taro
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Tachibana Taro
Department Of Anatomy And Cell Biology Osaka University Medical School
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Igarashi Koichi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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