New Detection Method for Hydrogen Gas for Screening Hydrogen-Producing Microorganisms Using Water-Soluble Wilkinson's Catalyst Derivative(METHODS)
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概要
- 論文の詳細を見る
A water-soluble color indicator was developed for the effective screening of hydrogen-producing microorganisms. This indicator consists of a coloring agent and a water-soluble derivative of Wilkinson's catalyst. Wilkinson's catalyst, Tris(triphenylphosphine) rhodium chloride, had been developed as a catalyst for the hydrogenation of olefins. We used a sulfonate of the catalyst for the hydrogenation of coloring agent in an aqueous medium. Several coloring agents, such as methyl orange, methyl red sodium, neutral red and Evan's blue, dissolved in water together with the sulfonated catalyst showed a change in color when hydrogen gas was fed into the solution by sparging at room temperature. We confirmed that methyl orange was decolorized by biologically produced hydrogen, when the photosynthetic bacterial strain Rhodobacter capsulatus ST-410 was grown in a medium containing 0.6mM catalyst and 0.075mM methyl orange in test tubes of 5ml working volume.
- 社団法人日本生物工学会の論文
- 2006-09-25
著者
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Katsuda Tomohisa
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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Katsuda T
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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KATO Jyoji
Department of Surgery II, Nagoya City University Medical School
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Azuma Masayuki
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Ooshima Hiroshi
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Ooshima H
Osaka City Univ. Osaka Jpn
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Ooshima Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ooshima Hiroshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Katsuda Tomohisa
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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Kato J
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Kato Jyoji
Department Of Bioapplied Chemistry
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Azuma M
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma Masayuki
Second Department Of Oral And Maxillofacial Surgery Tokushima University School Of Dentistry
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Kato Jyoji
Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University
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