Production of Hydrogen by a Hydrogenase-Deficient Mutant of Rhodobacter capsulatus

概要

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The characteristics of Rhodobacter capsulatus ST410, a mutant of the wild strain B100 lacking hydrogenase activity, were investigated from the viewpoint of hydrogen production. When 30 mM DL-malate and 7 mM L-glutamate were used as carbon and nitrogen sources, respectively, in an argon atmosphere, a specific hydrogen evolution rate of 0.14 ml/h/mg-dry cells was obtained at 6600 lx and 33℃. The evolution rate strongly depended on the light intensity : the higher the light intensity, the larger the evolution rate became up to at least 6600 lx. R. capsulatus ST410 converted 60 mM malate to hydrogen at a yield of 68%, calculated as a percentage of the stoichiometric maximum for the complete conversion of the carbon source to H_2 and CO_2. On the other hand, when the wild strain was used under the same conditions, the yield was only 25%. R. capsulatus ST410 converted not only malate but also glucose and cellobiose to hydrogen with good yields(60% for 30mM glucose and 66% for 7.5 mM cellobiose). Ethanolamine was found to be a good nitrogen source, which permitted a large amount of hydrogen to be evolved and also depressed the cell growth to low levels.
社団法人日本生物工学会の論文
1998-05-25