Characterization of Continuous Refolding Apparatus Using Different Mixing Methods
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概要
- 論文の詳細を見る
Taylor-eddy and paddle type apparatus were applied for refolding of lysozyme in a continuous system. The number of theoretical mixing stages was measured for both the apparatus and the effect of flow rate and mixing rate on the axial dispersion of solutions flowing inside of a ceramic membrane tube were investigated. Refolding at different concentrations of lysozyme was performed by addition of denatured lysozyme into refolding buffers flowing through the inside of the tube. For studying the effect of addition, different addition methods of denatured lysozyme into refolding buffer were devised by using ceramic membranes, from the total surface of the membrane tube and partially upside ceramic membrane. Comparing the efficiencies of continuous refolding with those of batch method, higher refolding efficiencies were obtained in the continuous system at the same concentrations of lysozyme and urea. Therefore, by combining the merits of the fed-batch method and continuous system, the refolding efficiency can be improved as well as throughput of refolding process.
- 社団法人 化学工学会の論文
- 2002-10-01
著者
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Katsuda T
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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KATOH Shigeo
Graduate School of Science and Technology, Kobe University
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Katsuda Tomohisa
Graduate School of Science and Technology, Kobe University
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Katsuda Tomohisa
Graduate School Of Science And Technology Kobe University
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Katoh Yoshihiro
Graduate School Of Science And Technology Kobe University
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Noda Hideo
Kansai Chemical Engineering Co. Ltd.
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Katoh S
Kobe Univ. Kobe Jpn
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Katoh Shigeo
Graduate School Of Engineering Department Of Chemical Science And Engineering Kobe University:depart
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Farshbaf Mahmood
Graduate School Of Science And Technology Kobe University
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FARSHBAF MAHMOUD
Graduate School of Science and Technology, Kobe University
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UDAKA TARO
Graduate School of Science and Technology, Kobe University
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Udaka Taro
Graduate School Of Science And Technology Kobe University
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