Effects of Ethanolamine as a Nitrogen Source on Hydrogen Production by Rhodobacter capsulatus
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概要
- 論文の詳細を見る
Ethanolamine was examined as a nitrogen source in the production of hydrogen by Rhodobacter capsulatus ST-410,a hydrogenase-deficient mutant of the strain B-100. It was found that ethanolamine supports cell growth as the sole nitrogen source and permits a large amount of hydrogen evolution, detected at 138 μmol/mlculture from 3.5 mM ethanolamine and 30 mM DL-malate. The amount corresponded to a stoichiometric yield of 77% and was close to that obtained from 7.0 mM L-glutamate and 30 mM DL-malate. The hydrogen evolution rate per unit biomass (cells) was higher than that with L-glutamate, and the cells grown with ethanolamine had higher nitrogenase activity than the cells grown with L-glutamate. In terms of bioconversion of cellulosic and hemicellulosic biomass to hydrogen, D-glucose, D-xylose, and D-cellobiose were tested as substrates. The results indicated that those sugars permit a large evolution of hydrogen through cultivation with ethanolamine as a nitrogen source. For instance, the cells grown with 3.5 mM ethanolamine evolved hydrogen of 289 μmol/ml-culture (80% yield) from 30 mM D-glucose under a controlled pH of 6.4 to 6.9.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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Katsuda Tomohisa
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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Katsuda T
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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KATO Jyoji
Department of Surgery II, Nagoya City University Medical School
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Azuma Masayuki
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Ooshima Hiroshi
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Ooshima H
Osaka City Univ. Osaka Jpn
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Ooshima Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ooshima Hiroshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Katsuda Tomohisa
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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Kato J
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Kato Jyoji
Department Of Bioapplied Chemistry
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Azuma M
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma Masayuki
Second Department Of Oral And Maxillofacial Surgery Tokushima University School Of Dentistry
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TAKAKUWA Susumu
Department of Natural Science, Kyoto Women's University
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Takakuwa Susumu
Department Of Natural Science Kyoto Women's University
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Takakuwa Susumu
Department Of Education Kyoto Women's University
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Kato Jyoji
Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University
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