Production of High Concentration Glucose-1-Phosphate by Immobilized Phosphorylase
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概要
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The continuous production of Glucose-1-phosphate (G-1-P) using immobilized potato phosphorylase was investigated. Potato phosphorylase was immobilized on a strongly basic anion-exchange resin, HPA-25 through contact of the resin with potato juice or wastewater of a potato-starch factory. The immobilization was highly selective for phosphorylase among proteins contained in potato juice and the wastewater. The immobilized phosphorylase could be used for the continuous G-1-P production over one year without significant deactivation. By using a 100-L column reactor packed with immobilized phosphorylase of 14.1 U·mL-carrier–1 at the superficial linear velocity of 0.034 m·h–1 and the space velocity of 3.1 × 10–9 m3·U–1·h–1, 185 mM G-1-P, which is the maximum concentration potentially expected from 516 mM dextrin and 2000 mM phosphate, was continuously produced.
- 社団法人 化学工学会の論文
- 2009-03-01
著者
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SAKATA Masaru
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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Ooshima Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ooshima Hiroshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Sakata Masaru
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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大嶋 寛
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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KAWAI Takashi
Processing Development Research Laboratories, Kao Corp.
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KAYANE Shigeto
Research & Development Division, Kao Corp.
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Kayane Shigeto
Research & Development Division Kao Corp.
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Kawai Takashi
Processing Development Research Laboratories Kao Corp.
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