Characterization of a Saccharomyces cerevisiae Mutant with Pseudohyphae and Cloning of a Gene Complementing the Mutation(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Screening for morphological mutants of a haploid strain of Saccharomyces cerevisiae was done on the basis of their cell-shape on a solid medium containing isoamyl alcohol, which causes cell elongation, to obtain information on the morphogenesis. Mutant J19, which had pseudohyphae in liquid medium even in the absence of isoamyl alcohol, had many elongated cells. Few reports exist of haploid cells growing as pseudohyphae in liquid culture. Cell-wall analysis showed that J19 had ordinary amounts of alkali-insoluble glucan and chitin, but that isoamyl alcohol in the medium caused structural changes in the cell wall. Addition of a DNA fragment that included the wild-type SCL1 gene to J19 complemented its morphological phenotype. Sequencing of J19 SCL1 showed that the glycine at position 226 in the Sel1 protein had been replaced by asparatic acid, suggesting that this mutation in the protein, a subunit of proteasomes, may be involved in the morphological change.
- 社団法人日本農芸化学会の論文
- 2003-03-23
著者
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Azuma Masayuki
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Ooshima Hiroshi
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Ooshima H
Osaka City Univ. Osaka Jpn
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Ooshima Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Ooshima Hiroshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Igarashi K
Dep. Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univ.
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Igarashi Koichi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Igarashi Koichi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Igarashi Koichi
Biology Research Laboratories:research And Development Division:tekada Chemical Industries Ltd.
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MANEESRI JARUWAN
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City Universit
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TORII Satoshi
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City Universit
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Torii Satoshi
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University
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Igarashi K
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma M
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Azuma Masayuki
Second Department Of Oral And Maxillofacial Surgery Tokushima University School Of Dentistry
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Maneesri Jaruwan
Department Of Applied And Bioapplied Chemistry Graduate School Of Engineering Osaka City University:
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Igarashi Koichi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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