Isolation of Enterococcus hirae Mutant Deficient in Low-affinity Potassium Uptake at Alkaline pH(Microbiology & Fermentation Technology)

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概要

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• We here isolated an Enterococcus hirae mutant unable to grow well at pH 10. The influx rate calculated from steady-state ^<42>K^+/K^+ exchange and the intracellular K^+ concentration of the mutant were reduced to 53 and 55% of those of the wild-type, respectively. The activities of two high-affinity K^+ uptake systems, KtrI and KtrII, were normal in the mutant, but the kinetics of net K^+ uptake at pH 10 indicated that a low-affinity K^+ uptake with a K_m of about 20 mM (Kawano, M, Abuki, R, Igarashi, K, Kakinuma, Y. (2001) Arch. Microbiol. 175 : 41-45), which were seen in the wild-type, was deficient in this mutant.

• 社団法人日本農芸化学会の論文
• 2002-07-23

著者

• IGARASHI Kazuei

  Graduate School of Pharmaceutical Sciences, Chiba University

• KAKINUMA Yoshimi

  Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University

• IKAWA-KITAYAMA Kaori

  Tochigi Research Laboratories of Kao Corporation

• Igarashi Kaori

  Graduate School Of Human Life Sciences Showa Women's University

• Igarashi Kazuei

  Graduate School Of Pharmaceutical Sciences Chiba University

• Kakinuma Yoshimi

  Dep. Of Applied Bioresource Sci. Fac. Of Agriculture Ehime Univ.

• Kakinuma Yoshimi

  Faculty Of Agriculture Ehime University

• Igarashi K

  Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

• KAWANO Miyuki

  Graduate School of Pharmaceutical Sciences, Chiba University

• Kawano Miyuki

  Graduate School Of Pharmaceutical Sciences Chiba University

• Kakinuma Y

  Integrated Center For Sciences (incs) Ehime University

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