Indispensable Glutamic Acid Residue-139 of NtpK Proteolipid in the Reaction of Vacuolar Na^+-Translocating ATPase in Enterococcus hirae
スポンサーリンク
概要
- 論文の詳細を見る
Enterococcus hirae vacuolar ATPase catalyzes translocation of Na^+ or Li^+ coupled with ATP hydrolysis. It is suggested that the glutamic acid residue (Glu139) of NtpK proteolipid subunit of this multisubunit enzyme is the binding site of these ions for translocation. Here we established a complementation system for the ntpK gene with its deletion mutant, and found that the ATPase activity disappeared upon replacement of Glu139 by aspartic acid. The sidechain length of this acidic residue of NtpK is thus important for this ATPase reaction.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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山登 一郎
Department Of Biological Science And Technology Tokyo University Of Science
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山登 一郎
東京理科大基礎工
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KAKINUMA Yoshimi
Faculty of Agriculture, Ehime University
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YAMATO Ichiro
Department of Materials Science and Engineering, Kochi National College of Technology
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KAKINUMA Yoshimi
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
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Igarashi Kazuei
Faculty of Pharmaceutical Science,Chiba University
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Igarashi Kazuei
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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TAKASE Kazuma
Lab. of Seeds Finding Technology, Eisai Co., Ltd.
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TAKASE Kazuma
Department of Biological Science and Technology, Science University of Tokyo
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Kakinuma Yoshimi
Dep. Of Applied Bioresource Sci. Fac. Of Agriculture Ehime Univ.
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Kakinuma Yoshimi
Faculty Of Agriculture Ehime University
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Igarashi K
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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Yamato Ichiro
Department Of Biological Science And Technology Science University Of Tokyo
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Takase Kazuma
Lab. Of Seeds Finding Technology Eisai Co. Ltd.
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Kakinuma Y
Integrated Center For Sciences (incs) Ehime University
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Igarashi Kazuhiko
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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Kakinuma Yoshimi
Faculty Agri, Ehime Univ.
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