Purification and Properties of a Low-molecular-weight, High-alkaline Pectate Lyase from an Alkaliphilic Strain of Bacillus
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概要
- 論文の詳細を見る
A low-molecular-weight, high-alkaline pectate lyase (pectate transeliminase, EC 4.2.2.2) was found in an alkaline culture of Bacillus sp. strain KSM-P15,purified to homogeneity, and crystallized. The enzyme had a relative molecular weight of approximately 20,300 as measured by sedimentation equilibrium, with a sedimentation coefficient (s^O_<20,w>) of 1.73S. It was a basic protein with an isoelectric point of pH 10.3,and the α-helical content was only 6.6%. In the presence of Ca^<2+> ions, the enzyme degraded polygalacturonic acid in a random manner to yield 4,5-unsatu-rated oligo-galacturonides and had its optimal activity around pH 10.5 and 50-55℃. It also had a protopectinaselike activity on cotton fibers. The N-terminal amino acid sequences of the intact protein (28 amino acids) and its two lysyl endopeptidase-cleaved peptide fragments (8 and 12 amino acids) had very low sequence similarity with pectate lyases reported to date. These results strongly suggest that the pectate lyase of Bacillus sp. strain KSM-P15 may be a novel enzyme and belongs in a new family.
- 社団法人日本農芸化学会の論文
- 1999-01-23
著者
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Hatada Y
Japan Agency For Marine‐earth Sci. And Technol. (jamstec) Yokosuka Jpn
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Hatada Yuji
Tochigi Research Laboratories Of Kao Corporation
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Ozawa T
Univ. Tsukuba Ibaraki Jpn
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Hayashi Y
Research Institute For Food Science Kyoto University
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Koike K
Faculty Of Pharmaceutical Sciences Toho University
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Koike Kenzo
Tochigi Research Laboratories, Kao Corporation
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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KOBAYASHI Tohru
Tochigi Research Laboratories of Kao Corporation
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HIGAKI Norihiko
Tochigi Research Laboratories of Kao Corporation
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SUZUMATSU Atsushi
Tochigi Research Laboratories of Kao Corporation
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HAKAMADA Yoshihiro
Tochigi Research Laboratories of Kao Corporation
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YOSHIMATSU Tadashi
Tochigi Research Laboratories of Kao Corporation
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OZAWA Tadahiro
Tochigi Research Laboratories of Kao Corporation
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Ozawa T
Tochigi Res. Lab. Kao Corp. Tochigi Jpn
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Koike K
Tochigi Research Laboratories Kao Corporation
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Ito S
National Industrial Research Institute
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Hayashi Y
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science Kyoto Pharmaceut
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Kobayashi Tohru
Tochigi Research Laboratories Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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