Cloning and Nucleotide Sequence of a hsp70 Gene from Streptomyces griseus
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概要
- 論文の詳細を見る
The cultivation of Streptomyces griseus 2247 at the growth-limited temperature (37℃) or in liquid medium containing 5% ethanol (toxic for growth) revealed the presence of heat-induced proteins in the total cellular proteins. Among them, a 70 kDal protein was isolated and its N-terminal amino acid sequence was determined. The 70 kDal protein possessed a possible ATP-binding site in the N-terminus, which was conserved among the HSP70 family. A DNA fragment encoding the HSP70 homologue was isolated from a genomic library of S. griseus 2247 strain using an oligonucleotide probe based on the N-terminal amino acid sequence of the 70 kDal protein. DNA sequence analysis of the cloned gene revealed an open reading frame consisting of 618 amino acid residues. The deduced amino acid sequence is highly homologous to the HSP70 family proteins; it is 59.8% identical to Clostridium perfringens HSP70,59.7% to the Bacillus megaterium DnaK protein, 58.4% to the Methanosarcina mazei DnaK protein, 58.1% to Synechocystis HSP70,52.8% to the DnaK protein of Escherichia coli, and about 50% to some of the mitochondrial heat shock proteins. The cloned gene could encode the HSP70 of S. griseus.
- 社団法人日本生物工学会の論文
- 1994-05-25
著者
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Hatada Y
Japan Agency For Marine‐earth Sci. And Technol. (jamstec) Yokosuka Jpn
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Hatada Yuji
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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KINASHI Haruyasu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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SHINKAWA Hidenori
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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HAKAMADA Yoshihiro
Tochigi Research Laboratories of Kao Corporation
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NIMI Osamu
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Nimi O
Hiroshima Bunkyo Women's College
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Nimi Osamu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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KAWAMOTO KAZUYUKI
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kawamoto Kazuyuki
Dept. Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kinashi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shinkawa H
Hiroshima Univ. Higashi Hiroshima Jpn
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Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Shinkawa Hidenori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kinashi Haruyasu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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