Primary Characterization of Curved DNA Segments Cloned from Streptomyces DNA with Extremely High G/C-contents(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Until recently, it was assumed that any short DNA segment could be regarded as a straight rod. Many instances, however, have been reported in which the helical axis was curved. In this study, to gain a general insight into the structural features of sequence-directed DNA curvatures, we constructed a set of plasmids carrying curved DNA segments, which were randomly cloned from Streptomyces total DNA with extremely high G/C-contents. The results of primary characterization of these curved DNA segments are presented. Although the cloned DNA segments had high G/C-contents, in all cases, several homopolymeric [dA] and [dT] stretches were found to be clustered around the centres of the determined nucleotide sequences. Furthermore, the clustered [dA] and [dT] stretches were located nearly in phase with the DNA helical screw. One of the DNA segments characterized in this study appeared to be derived from the giant linear plasmid, SCP1, and another from the circular fertility plasmid, SCP2.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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MIZUNO Takeshi
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoy
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UEKI Nobuhide
Helix Research Institute
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Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Mizuno Takeshi
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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UEKI Nobuhide
Laboratory of Microbiology, School of Agriculture, Nagoya University
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Kinashi Haruyasu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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MIZUNO Takeshi
Department of Biochemistry, State University of New York at Stony Brook
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Mizuno Takeshi
Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
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