Improvement of Transformation System in Streptomyces Using a Modified Regeneration Medium
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概要
- 論文の詳細を見る
For improvement of the regeneration frequency of Streptomyces griseus 2247 protoplasts, the effects of growth phase and components of the regeneration medium on the frequency were investigated. Protoplasts prepared from transition-phase mycelia of S. griseus 2247 exhibited the highest regeneration frequency. An improved regeneration medium designated R1M was estabilished by supplementing Polypepton and yeast extract which were shown to increase the S. griseus 2247 growth rate to R1 previously developed for S. griseus. The regeneration frequencies of S. griseus 2247 and its pleiotropic mutant HT-3 were greatly increased to 87.5% and 92.5%, respectively, on R1M medium. Using this protoplast-regeneration system, the transformation efficiency for the plasmid pRES18 was increased to 10^6 transformants per μg DNA. R1M medium was also effective for the protoplast regeneration of some other antibiotic-producing streptomycete species.
- 社団法人日本生物工学会の論文
- 1997-03-25
著者
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ISHIKAWA Jun
Department of Bioactive Molecules, National Institute of Infectious Diseases
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KINASHI Haruyasu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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SHINKAWA Hidenori
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Zhang Hui
Department Of Cardiovascular Respiratory And Metabolic Medicine Graduate School Of Medicine Kagoshim
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Ishikawa J
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Ishikawa Jun
大阪大学 医学系研究科心臓血管外科
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Zhang H
Life Sci. Coll. Shandong Univ. Shandong Province Chn
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NIMI OSAMU
Hiroshima Bunkyo Women's College
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Nimi O
Hiroshima Bunkyo Women's College
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Nimi Osamu
Hiroshima Bunkyo Women's College
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Ishikawa Jun
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Kinashi H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shinkawa H
Hiroshima Univ. Higashi Hiroshima Jpn
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Zhang H
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Zhang H
Carnegie Mellon Univ. Pittsburgh Usa
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Shinkawa Hidenori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kinashi Haruyasu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Zhang Hui
Department Of Biomolecular Recognition And Ophthalmology Yamaguchi University School Of Medicine
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