Physical Mapping of the Linear Plasmid pSLA2-L and Localization of the eryAI and actI Homologs
スポンサーリンク
概要
- 論文の詳細を見る
The 200-kb linear plasmid pSLA2-L was suggested to be involved in the production of lankamycin and lankacidin in Streptomyces rochei 7434AN4. In this study, we have constructed a physical map for 23 PstI fragments of pSLA2-L, the sum of which was 206 kb. Detailed restriction maps for both ends of pSLA2-L revealed the presence of terminal inverted repeats, the size of which was found to be 2.1 kb by cloning and sequencing of the end-points. Hybridization experiments using two polyketide biosynthetic genes, eryAI and actI, located their homologous regions on PstI fragments A and I, respectively.
- 1998-10-23
著者
-
FUJII Shingo
Department of Gynecology and Obstetrics, Graduate School of Medicine, Kyoto University
-
KINASHI Haruyasu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
-
HATANI Akira
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
-
KUROKAWA Toru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
-
SHINKAWA Hidenori
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
-
Hatani Akira
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Fujii Shingo
Department Of Gynecology And Obstetrics Graduate School Of Medicine Kyoto University
-
Fujii Shingo
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Kurokawa T
Kochi Gakuen Coll. Kochi Jpn
-
Kurokawa Toru
Department Of Child Neurology National Center Hospital For Mental Nervous And Muscular Disorders Nat
-
Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Shinkawa Hidenori
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
-
Shinkawa Hidenori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
-
Kinashi Haruyasu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
-
Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
関連論文
- Adnexal Masses Caused by Pelvic Inflammatory Disease : MR Appearance
- Paraneoplastic syndromes associated with ovarian neoplasms
- Clinical value of FDG-PET in the follow up of post-operative patients with endometrial cancer
- Physical Mapping of the Linear Plasmid pSLA2-L and Localization of the eryAI and actI Homologs
- Significant Increase in Maternal Plasma Leptin Concentration in Induced Delivery : A Possible Contribution of Pro-inflammatory Cytokines to Placental Leptin Secretion
- Free-to-Total Leptin Ratio in Maternal Plasma is Constant Throughout Human Pregnancy
- Significant increase in maternal plasma leptin concentration during induced labor : A possible contribution of pro-inflammatory cytokines to placental leptin secretion(Session20 Peninatology5)
- Protoplasting, Regeneration and Transformation of Rare Actinomycetes
- Improvement of Transformation System in Streptomyces Using a Modified Regeneration Medium
- The Relationship between Maternal Plasma Leptin Levels and Fetal Growth Restriction
- Nucleotide Sequence of a Principal Sigma Factor Gene (hrdB) of Streptomyces griseus
- Cloning and Nucleotide Sequence of a hsp70 Gene from Streptomyces griseus
- Induction of Pleiotropic Mutation in Streptomyces griseus by Incubation under Stress Conditions for Mycelial Growth
- Crucial role of FOXP3 in the development and function of human CD25^+CD4^+ regulatory T cells
- Successful management of a pregnant woman with heterozygous protein C deficiency using activated protein C concentrate
- Purification and characterization of RNA Polymerase holoenzyme (Eσ_B) from Vegetative-Phase Mycelia of Streptomyces griseus
- Intractable recurrent cervical cancer with pelvic bone involvement successfully treated with external hemipelvectomy
- Spontaneous regression of congenital cystic adenomatoid malformation of the lung : Longitudinal examinations by magnetic resonance imaging
- A case of successful management of maternal septic shock with multiple organ failure following amniocentesis at midgestation
- Chromosomal Circularization in Streptomyces griseus by Nonhomologous Recombination of Deletion Ends(Biochemistry & Molecular Biology)
- Role of salvage cytoreductive surgery in the treatment of patients with recurrent ovarian cancer after platinum-based chemotherapy
- IS-4 Loss of expression of Cold-inducible RNA binding protein (CIRP) in endometrial carcinoma
- Regulation of Lankamycin Biosynthesis in Streptomyces rochei by Two SARP Genes, srrY and srrZ
- Laminin and fibronectin concentrations of the follicular fluid correlate with granulosa cell luteinizaiton and oocyte quality
- Fetal Undernutrition Programs Adipose Tissue Metabolism in Adulthood(Perinatology 2)
- Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor
- IS-130 The Increased Prostacyclin Production in Human Myometrium during Pregnancy Is due to Augmented Expressions of Cyclooxygenase-1(COX-1)and Prostacyclin Synthase(PGIS)
- IS-93 The Free to Total Leptin Ratio in the Plasma of Pregnant Women Remains Constant during Pregnancy and Labor
- pSLA2-M of Streptomyces rochei Is a Composite Linear Plasmid Characterized by Self-Defense Genes and Homology with pSLA2-L
- Clinical features of epilepsy with pervasive developmental disorder
- Extensive Mutational Analysis of Modular-Iterative Mixed Polyketide Biosynthesis of Lankacidin in Streptomyces rochei
- Analysis of Modular-iterative Mixed Biosynthesis of Lankacidin by Heterologous Expression and Gene Fusion
- Ascitic Positive Cytology and Intraperitoneal Metastasis in Ovarian Dysgerminoma
- Olivopontocerebellar atrophy of neonatal onset with muscle hypertonia in two siblings
- Identification of a Gene Cluster of Polyether Antibiotic Lasalocid from Streptomyces lasaliensis
- Membrane-Bound Cell Surface Peptidases in Reproductive Organs
- Ovarian Pregnancy Associated with Microscopic Decidualized Endometriosis of the Ovary : Report of a Case
- Prediction of Histopathologic Features and Proliferative Activity of Uterine Leiomyoma by Magnetic Resonance Imaging Prior to GnRH Analogue Therapy : Correlation between T2-Weighted Images and Effect of GnRH Analogue
- Transcription Apparatus and Its Relatives in Streptomyces
- Gene disruption analysis of two glycosylation steps in lankamycin biosynthesis in Streptomyces rochei
- Use of Intravenous Adenosine Triphosphate (ATP) to Terminate Supraventricular Tachycardia in a Pregnant Woman with Wolff-Parkinson-White Syndrome
- Primary Characterization of Curved DNA Segments Cloned from Streptomyces DNA with Extremely High G/C-contents(Microbiology & Fermentation Industry)
- Changes in the Serum Levels of Human Chorionic Gonadotropin and the Pulsatility Index of Uterine Arteries during Conservative Management of Retained Adherent Placenta
- pSLA2-M of Streptomyces rochei Is a Composite Linear Plasmid Characterized by Self-Defense Genes and Homology with pSLA2-L
- Characterization of the dnaK Locus in Streptomyces griseus
- Tissue-type plasminogen activator-assisted drainage of suprachoroidal hemorrhage showing a kissing configuration
- Isolation of Borrelidin as a Phytotoxic Compound from a Potato Pathogenic Streptomyces Strain
- Giant linear plasmids in Streptomyces : a treasure trove of antibiotic biosynthetic clusters
- Antibiotic production, linear plasmids and linear chromosomes in Streptomyces