pSLA2-M of Streptomyces rochei Is a Composite Linear Plasmid Characterized by Self-Defense Genes and Homology with pSLA2-L
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概要
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The 113,463-bp nucleotide sequence of the linear plasmid pSLA2-M of Streptomyces rochei 7434AN4 was determined. pSLA2-M had a 69.7% overall GC content, 352-bp terminal inverted repeats with 91% (321/352) identity at both ends, and 121 open reading frames. The rightmost 14.6-kb sequence was almost (14,550/14,555) identical to that of the coexisting 211-kb linear plasmid pSLA2-L. Adjacent to this homologous region an 11.8-kb CRISPR cluster was identified, which is known to function against phage infection in prokaryotes. This cluster region as well as another one containing two large membrane protein genes (orf78 and orf79) were flanked by direct repeats of 194 and 566 bp respectively. Hence the insertion of circular DNAs containing each cluster by homologous recombination was suggested. In addition, the orf71 encoded a Ku70/Ku80-like protein, known to function in the repair of double-strand DNA breaks in eukaryotes, but disruption of it did not affect the radiation sensitivity of the mutant. A pair of replication initiation genes (orf1-orf2) were identified at the extreme left end. Thus, pSLA2-M proved to be a composite linear plasmid characterized by self-defense genes and homology with pSLA2-L that might have been generated by multiple recombination events.
- 社団法人 日本農芸化学会の論文
著者
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ENDO Satoru
Quantum Energy Applications, Graduated School of Engineering, Hiroshima University
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ARAKAWA Kenji
Department of Chemistry, Box 351700, University of Washington
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KINASHI Haruyasu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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KUROKAWA Toru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Endo Satoru
Quant. Energ. Applic. Grad. Sch. Eng. Hiroshima Univ.
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Kurokawa Toru
Department Of Child Neurology National Center Hospital For Mental Nervous And Muscular Disorders Nat
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YANG Yingjie
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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TAKAHAMA Yoshifumi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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NINDITA Yosi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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MOCHIZUKI Susumu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Kinashi Haruyasu
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Arakawa Kenji
Department Of Chemistry Box 351700 University Of Washington
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Takahama Yoshifumi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Nindita Yosi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kinashi Haruyasu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kurokawa Toru
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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