Geranylgeranyl Diphosphate Synthase from Scoparia dulcis and Croton sublyratus. Plastid Localization and Conversion to a Farnesyl Diphosphate Synthase by Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
cDNAs encoding geranylgeranyl diphosphate synthase(GGPPS)of two diterpene-producing plants, Scoparia dulcis and Croton sublyratus, have been isolated using the homology-based polymerase chain reaction(PCR) method. Both clones contained highly conserved aspartate-rich motifs(DDXX(XX)D)and their N-terminal residues exhibited the characteristics of chloroplast targeting sequence. When expressed in Escherichia coli, both the full-length and truncated proteins in which the putative targeting sequence was deleted catalyzed the condensation of farnesyl diphosphate and isopentenyl diphosphate to produce geranylgeranyl diphosphate(GGPP). The structural factors determining the product length in plant GGPPSs were investigated by constructing S.dulcis GGPPS mutants on the basis of sequence comparison with the first aspartate-rich motif(FARM) of plant farnesyl diphosphate synthase. The result indicated that in plant GGPPSs small amino acids, Met and Ser, at the fourth and fifth positions before FARM and Pro and Cys insertion in FARM play essential roles in determination of product length. Further, when a chimeric gene comprised of the putative transit peptide of the S.dulcis GGPPS gene and a green fluorescent protein was introduced into Arabidopsis leaves by particle gun bombardment, the chimeric protein was localized in chloroplasts, indicating that the cloned S.dulcis GGPPS is a chloroplast protein.
- 社団法人日本薬学会の論文
- 2001-02-01
著者
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Niwa Yasuo
Plant Science Center Riken(the Institute Of Physical And Chemical Research)
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Niwa Yasuo
Lab. Of Plant Cell Technol. Graduate School Of Nutritional And Environmental Sciences Univ. Of Shizu
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Niwa Yasuo
Laboratory Of Plant Cell Technology And Coe Program In The 21st Century Graduate School Of Nutrition
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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Niwa Y
Laboratory Of Plant Cell Technology Graduate School Of Nutritional And Environmental Sciences Univer
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SITTHITHAWORN Worapan
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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KOJIMA Naoe
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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VIROONCHATAPAN Ekapop
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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SUH Dae-Yeon
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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IWANAMI Naoko
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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HAYASHI Toshimitsu
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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NOJI Masaaki
Laboratory of Molecular Biology and Biotechnology, Research Center of Medicinal Resources, Faculty o
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SAITO Kazuki
Laboratory of Molecular Biology and Biotechnology, Research Center of Medicinal Resources, Faculty o
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SANKAWA Ushio
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceautical Sciences University Of Tokyo
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Sitthithaworn Worapan
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Noji M
Laboratory Of Molecular Biology And Biotechnology Research Center Of Medicinal Resources Faculty Of
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Iwanami N
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Iwanami Naoko
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Hayashi Toshimitsu
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Suh Dae-yeon
Department Of Chemistry And Biochemistry University Of Regina
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Hayashi T
Faculty Of Pharmaceutical Sciences Josai University
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Kojima N
Faculty Of Pharmaceutical Science Hokuriku University
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Hirooka Takashi
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Saito Kazuki
Laboratory Of Molecular Biology And Biotechnology Research Center Of Medicinal Resources Faculty Of
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Saito Kazuki
Laboratory Of Molecular Biology And Biotechnology Research Center Of Medicinal Resources Faculty Of
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Viroonchatapan Ekapop
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Suh Dae-yeon
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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