Purification and Characterization of Sulochrin Oxidase from Penicillium frequentans
スポンサーリンク
概要
- 論文の詳細を見る
Sulochrin oxidase, an enzyme catalyzing regio- and stereospecific phenol oxidative coupling reaction to form (+)-bisdechlorogeodin from sulochrin, was isolated from Penicillium frequentans CMI 96659. By chromatographies on DEAE-cellulose, hydroxyapatite, Phenyl-Sepharose, Mono P, Mono Q, and HPLC gel filtration columns, sulochrin oxidase was purified to apparent homogeneity. The purified enzyme had a molecular weight of 230 K as estimated by gel filtration and 110 K as estimated by sodium dodecylsulfate-polyacrylamide gel electrophoresis under denaturing conditions, suggesting that the active enzyme was a homodimer. The enzyme showed pI 4.0 and an optimum pH of 6. The enzyme activity was strongly inhibited by the copper-chelating reagent, diethyldithiocarbamate, and did not recover its full activity even after removing the inhibitor by dialysis. However, enzyme activity was fully restored by the addition of Cu^<2+>. Thus, sulochrin oxidase is considered to be a copper protein. The enzyme showed high substrate specificity for benzophenone compounds such as sulochrin and dihydrogeodin.
- 公益社団法人日本薬学会の論文
- 1996-01-15
著者
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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三川 潮
東大 薬
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SANKAWA Ushio
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceautical Sciences University Of Tokyo
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Fujii Isao
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Yoshida Yuki
Showa Pharmaceutical University
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Faculty Of Pharmaceautical Sciences University Of Tokyo
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Yoshida Y
Yakult Central Inst. For Microbiological Res.
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HUANG Kexue
Faculty of Pharmaceutical Sciences, The University of Tokyo
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YOSHIDA Yasunori
Faculty of Pharmaceutical Sciences, The University of Tokyo
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MIKAWA Kazunobu
Faculty of Pharmaceutical Sciences, The University of Tokyo
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Huang Kexue
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Mikawa Kazunobu
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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