MOLECULAR CLONING OF cDNA ENCODING RAT 2,3-OXIDOSQUALENE : LANOSTEROL CYCLASE
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA encoding rat 2,3-oxidosqualene : lanosterol cyclase, the enzyme responsible for the complex cyclization/rearrangement step in sterol biosynthesis, was cloned by extensive application of PCRs. Oligonucleotide primers were designed in sense and anti-sense directions corresponding to internal peptide sequences of purified enzyme. Successive PCRs with all possible combinations of these primers yielded a 178-bp fragment, and based on its sequence full nucleotide sequence of cDNA was obtained by a "rapid amplification of cDNA ends"(RACE) method.
- 社団法人日本薬学会の論文
- 1995-01-15
著者
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三川 潮
Faculty of Pharmaceutical Sciences, University of Tokyo
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海老塚 豊
東大院薬
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海老塚 豊
Faculty of Pharmaceutical Sciences, The University of Tokyo
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渋谷 雅明
Faculty of Pharmaceutical Sciences, The University of Tokyo
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceuical Sciences The University Of Tokyo
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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三川 潮
東大 薬
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草野 昌行
東大・院・薬
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草野 昌行
Faculty of Pharmaceutical Sciences, The University of Tokyo
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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