MOLECULAR CLONING OF cDNA ENCODING HUMANLANOSTEROL SYNTHASE
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概要
- 論文の詳細を見る
A cDNA encoding human lanosterol synthase, the enzyme responsible for the backbone formation step in sterol biosynthesis, was cloned by extensive application of PCRs. Five degenerate oligonucleotide primers (139S, 440S, 528A, 575A and 712A) corresponding to the homologous amino acid sequences among the known 2,3-oxidosqualene cyclase (OSC) were designed. PCR with one pair (440S and 528A) of five primers yielded a 285-bp fragment. PCRs with the primers based on the obtained fragment and the degenerate primers (139S and 712A) gave longer fragments. Finally, full nucleotide sequence of cDNA was obtained by a "rapid amplification of cDNA ends"(RACE) method.
- 社団法人日本薬学会の論文
- 1995-10-15
著者
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三川 潮
Faculty of Pharmaceutical Sciences, University of Tokyo
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海老塚 豊
東大院薬
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海老塚 豊
Faculty of Pharmaceutical Sciences, The University of Tokyo
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渋谷 雅明
Faculty of Pharmaceutical Sciences, The University of Tokyo
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceuical Sciences The University Of Tokyo
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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三川 潮
東大 薬
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成 忠基
Faculty of Pharmaceutical Sciences, The University of Tokyo
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成 忠基
韓国全南大薬
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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