ISOLATION, SEQUENCE AND BACTERIAL EXPRESSION OF A cDNA FOR CHALCONE SYNTHASE FROM THE CULTURED CELLS OF PUERARIA LOBATA
スポンサーリンク
概要
- 論文の詳細を見る
cDNA clones for chalcone synthase (CHS) of Pueraria lobata cultured cells were isolated by screening the cDNA library using CHS cDNA of Phaseolus vulgaris as a probe. Analysis of nucleotide sequences of the cloned cDNA revealed a 1170-bp open reading frame that encoded a 390-amino acid polypeptide with an Mr of 43,000. The full-length cDNA was cloned into the expression vector pT7-7. CHS activity was found in the crude extracts of transformed E. coli after induction and two protein bands of ca. 43 and 34 kd were hybridized with anti-persley CHS antiserum.
- 社団法人日本薬学会の論文
- 1991-07-25
著者
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三川 潮
Faculty of Pharmaceutical Sciences, University of Tokyo
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野口 博司
静岡県大薬
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野口 博司
静岡県立大学薬学部
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袴塚 高志
東大薬
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袴塚 高志
Faculty of Pharmaceutical Sciences, The University of Tokyo
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野口 博司
Faculty of Pharmaceutical Sciences, The University of Tokyo
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海老塚 豊
Faculty of Pharmaceutical Sciences, The University of Tokyo
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中島 治
Faculty of Pharmaceutical Sciences, The University of Tokyo
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秋山 卓美
Faculty of Pharmaceutical Sciences, The University of Tokyo
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渋谷 雅明
Faculty of Pharmaceutical Sciences, The University of Tokyo
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceuical Sciences The University Of Tokyo
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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秋山 卓美
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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三川 潮
東大 薬
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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中島 治
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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野口 博司
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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