PURIFICATION OF 2,3-OXIDOSQUALENE / CYCLOARTENOL CYCLASE FROM PEA SEEDLINGS
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概要
- 論文の詳細を見る
2,3-Oxidosqualene / cycloartenol cyclase (E.C.5.4.99.8) is a membrane bound enzyme prevalent in the plant kingdom. It acts as a key enzyme in phytosterol biosynthesis. Cycloartenol cyclase was purified from pea seedlings in six steps as a soluble and homogeneous enzyme. The purified enzyme showed a single band in sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular weight of 55 K Da and had a Km value of 25 μM. cycloartenol cyclase requires Triton X-100 or deoxycholate for its highest activity.
- 社団法人日本薬学会の論文
- 1988-12-25
著者
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三川 潮
Faculty of Pharmaceutical Sciences, University of Tokyo
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阿部 郁朗
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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三川 潮
Faculty Of Pharmaceuical Sciences The University Of Tokyo
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海老 塚豊
Faculty of Pharmaceutical Sciences, the University of Tokyo
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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海老 塚豊
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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