cDNA and Genomic DNA Clonings of Chalcone Synthase from Pueraria lobata
スポンサーリンク
概要
- 論文の詳細を見る
Four cDNAs encoding chalcone synthase (CHS), the key enzyme in flavonoid biosynthesis, were isolated from Pueraria lobata cells challenged with yeast extract elicitor using bean CHS cDNA as a probe. The longest clone contained a complete open reading frame of 1170 bp which would predict a protein of about 43 kDa. The others were not full-length clones. Using isolated cDNA as a probe, Southern blot hybridization of genomic DNA fragments revealed the presence of multiple CHS genes in the P. lobata genome. We cloned and sequenced one CHS genomic clone, gCHS14,whose 5'untranslated region showed homology with the bean CHS gene CHS15 and included the several reported sequences characteristic of stress response.
- 公益社団法人日本薬学会の論文
- 1996-01-15
著者
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三川 潮
富山医薬大薬
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三川 潮
Faculty Of Pharmaceutical Sciences University Of Tokyo
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NOGUCHI Hiroshi
Faculty of Engineering, Kyushu University
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SANKAWA Ushio
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Sankawa U
International Research Center For Traditional Medicine
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Sankawa Ushio
(present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Sankawa Ushio
Faculty Of Pharmaceautical Sciences University Of Tokyo
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SHIBUYA MASAAKI
Faculty of Pharmaceutical Sciences, University of Tokyo
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Noguchi H
National Institute Of Agro-environmental Sciences
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HAKAMATSUKA Takashi
Faculty of Pharmaceutical Sciences, Tokyo Science University
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Hakamatsuka T
Faculty Of Pharmaceutical Sciences Tokyo Science University
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Faculty Of Pharmaceautical Sciences University Of Tokyo
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Shibuya Masaaki
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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NAKAJIMA Osamu
Faculty of Pharmaceutical Sciences, The University of Tokyo
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Hakamatsuka Takashi
Faculty Of Pharmaceutical Sciences Rikadai (tokyo University Of Science)
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Nakajima Osamu
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Noguchi H
Department Of Biology Faculty Of Science Chiba University
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Noguchi Hiroshi
Faculty Of Engineering Kyushu University
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Noguchi Hiroshi
Faculty Of Commerce University Of Marketing And Distribution Sciences
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