Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
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概要
- 論文の詳細を見る
We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.
- 社団法人 日本農芸化学会の論文
- 2008-02-23
著者
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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Suzuki Takamasa
Department Of Electronics Nagoya University:r&d Department Nippondenso Co. Ltd.
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Ishiguro Sumie
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Ishiguro S
Nagoya Univ. Nagoya Jpn
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Ishiguro Sumie
Dept. Botany Grad. Sch. Science Kyoto Univ.
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Ishiguro Sumie
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Takahashi N
Plant Functions Laboratory Riken
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Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Tanaka Katsunori
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
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Tanaka K
Tottori Univ. Tottori
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Nakamura K
Nagoya Univ. Nagoya Jpn
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
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NAKAMURA Kenzo
Department of Urology, University of Yamanashi
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Kimura Tetsuya
Department Of Clinical Retrovirology And Infectious Diseases Center For Aids Research Kumamoto Unive
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kimura Tetsuya
Dep. Of Sustainable Resource Sci. Graduate School Of Bioresources Mie Univ.
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Nakamura Kenzo
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Suzuki Toshiya
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Nakamura Shinya
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Takahashi Nobuyuki
Hokkaido University Of Education
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Nakano Takeshi
Plant Functions Lab. The Inst. Of Physical And Chemical Res.
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Nakano Takeshi
Plant Functions Lab.
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Nakamura Shinya
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Okayama University
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Kikuchi Takao
Coll. Environ. Health Azabu Univ.
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Nakamura Shinya
Department Of Chemistry University Of Tsukuba
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Nakamura Kenzo
Department Of Applied Biological Sciences Faculty Of Agricultural Sciences Nagoya University
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Tanaka Keiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Suzuki Takamasa
Department Of Applied Science Faculty Of Engineering Kyushu University
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Takahashi Nobuyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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Koyanagi Takashi
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Suzuki Takamasa
Department Of Biological Functions And Mechanisms Graduate School Of Bioagricultural Sciences Nagoya University
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Kimura Tetsuya
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
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Takahashi Noriko
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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NAKAGAWA Tsuyoshi
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
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