Genetic Analysis of chs1^+ and chs2^+ Encoding Chitin Synthases from Schizosaccharomyces pombe

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概要

• 論文の詳細を見る

• To explore the function of chitin in Schizosaccharomyces pombe, we have cloned chs1+ and chs2+, encoding putative chitin synthases, based on sequences in the Sanger Centre database. The synthetic lethal phenotype of the S. cerevisiae chs1 chs2 chs3 mutant was complemented by expression of S. pombe chs1+ or chs2+, indicating that both chs1+ and chs2+ in fact encode chitin synthase. The homothallic Δchs1 strain formed abnormal asci that contained 1, 2, or 3 spores, while the Δchs2 strain had no noticeable phenotype. The chs1 chs2 double disruptant looked similar phenotypically to the Δchs1 strain. The Chs2-GFP fusion protein predominantly localized at the septum after the septum was formed during vegetative growth. The level of chs2+ mRNA increased just before the septum was formed. Levels of Chs2-13Myc synthesis also changed during the cell cycle. Thus, chs1+ is required for proper spore formation, and chs2+ is perhaps involved in septum formation.

• 社団法人 日本農芸化学会の論文
• 2004-07-23

著者

• Takahashi N

  Plant Functions Laboratory Riken

• Tanaka K

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• KAWAMUKAI Makoto

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• MATSUDA Hideyuki

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• Tanaka Katsunori

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima

• Tanaka K

  Tottori Univ. Tottori

• NAKAGAWA Tsuyoshi

  Center for Integrated Research in Science, Shimane University

• Nakano Takeshi

  Riken Advanced Sci. Inst.

• MATSUO Yasuhiro

  Ariake National College of Technology

• Takahashi Nobutaka

  Frontier Research Program The Institute Of Physical And Chemical Research

• Takahashi Nobutaka

  Frontier Research Program The Institute Of Physical And Chemical Research (rlken)

• MATSUO Yasuhiro

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• Nakagawa Tetsuto

  Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra

• Nakagawa Tsuyoshi

  Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• NAKANO Takeshi

  The Institute of Physical and Chemical Research (RIKEN)

• Kawamukai Makoto

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Nakagawa T

  Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra

• Matsuo Yasuhiro

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Morita K

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Kawamukai M

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Takahashi Nobuyuki

  Hokkaido University Of Education

• Matsuda H

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda Hideyuki

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Nakagawa Tsuyoshi

  Center For Integrated Research In Science Shimane University

• Kawamukai Makoto

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Nakano Takeshi

  Plant Functions Lab. The Inst. Of Physical And Chemical Res.

• Nakano Takeshi

  Plant Functions Lab.

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Tanaka Keiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Nakagawa Tsuyoshi

  Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un

• Kawamukai Makoto

  Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive

• Takahashi Nobuyuki

  Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School

• Koyanagi Takashi

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Takahashi Noriko

  Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

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