Preparation of an Endosperm Protoplasts from a Dwarf Rice Variety and Transient Expression of Green-Fluorescent Protein
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概要
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Vital protoplasts were isolated from developing dwarf rice endosperms about 10 days post anthesis. A yield of 0.98x105±0.12 protoplasts was obtained from 10 developing grains. These protoplasts were viable for at least three days in the culture medium. Transient expression of the green-fluorescent protein (GFP) was detected after four hours of introduction of the GFP gene by the polyethylene glycol (PEG)-mediated DNA transfer. As expected, the fluorescence of GFP without the targeting sequence was observed diffusely in the cytoplasm. On the other hand, the fluorescence of the fusion protein for rice prolamin followed by the GFP was observed in the small particles and the ER-network. These endosperm protoplasts had no autofluorescence upon excitation with 490 nm (blue) light. The vital protoplasts obtained from this dwarf rice variety are very useful for the physiological, biochemical and molecular biology studies of rice endosperm.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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Morita Shigeto
Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni
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Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Tanaka K
Tottori Univ. Tottori
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Masumura Takehiro
Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.
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Morita S
Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni
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Takahashi Hideyuki
Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Japan
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Tanaka Kunisuke
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect
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MORITA Shigeto
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect
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MASUMURA Takehiro
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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Masumura T
Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center
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Tanaka Kunisuke
Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University
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Masumura Takehiro
Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University
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Kikuchi Takao
Coll. Environ. Health Azabu Univ.
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Takahashi Hideyuki
Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University
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Tanaka Keiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Koyanagi Takashi
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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MORITA Shigeto
Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University
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