Molecular Cloning and Characterization of a cDNA for an Iron-Superoxide Dismutase in Rice (Oryza sativa L.)

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概要

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• We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka K

  Tottori Univ. Tottori

• Masumura Takehiro

  Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MORITA Shigeto

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MASUMURA Takehiro

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• Kaminaka Hironori

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• TOKUMOTO Megumi

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• YOKOYAMA Hidefumi

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• Yokoyama H

  Tokyo Inst. Technol. Yokohama Jpn

• Yokoi H

  Tokyo Inst. Technol. Tokyo Jpn

• Yokoi Hideki

  Department Of Electrical And Electronic Engineering Graduate School Of Science And Engineering Tokyo

• Kaminaka Hironori

  Lab. Genetic Engineering Fac. Of Agr. Kyoto Pref. Univ.

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Masumura T

  Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center

• Yokoi H

  Graduate School Of Science And Engineering Tokyo Institute Of Technology

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Tokumoto Megumi

  Lab. Genetic Engineering. Dept. Agri. Kyoto Pref. Univ.

• Yokoi H

  Department Of Electrical And Electronic Engineering Graduete School Of Science And Engineering Tokyo

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• MORITA Shigeto

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

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