Separation of Specific Cells Using a Laser Micro-dissection System and Detection of mRNA in Developing Rice Endosperm Tissue

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概要

• 論文の詳細を見る

• Cloning of the expressing gene in the target cell is important for understanding the molecular mechanism of specific gene expression. Laser micro-dissection techniques allow the precise separation of a single target cell from morphologically distinct cells. We describe here an excellent method for separating a single cell or a cell cluster consisting of a few cells from a small target area of plant tissues. In rice endosperm tissues, the aleurone layer and starchy endosperm tissue are differentiated from a single endosperm mother cell. We succeeded in dissecting a specific cell area from endosperm and aleurone tissue (7 days after flowering). This information will be useful for studying cell differentiation and developing mechanisms of endosperm tissue. RT-PCR analysis for mRNAs for actin and glutelin confirmed the purity of the dissected cells.

• 日本植物細胞分子生物学会の論文
• 2002-06-01

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka K

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Tanaka K

  Tottori Univ. Tottori

• Masumura Takehiro

  Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MORITA Shigeto

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MASUMURA Takehiro

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• KURITA Akihiro

  Laboratory of Genetic Engineering, Graduate school of Agriculture, Kyoto Prefectural University

• OHYAMA Rie

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• Kubo A

  National Inst. For Environmental Studies Ibaraki Jpn

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Masumura T

  Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center

• Ohyama Rie

  Laboratory Of Genetic Engineering Faculty Of Agriculture Kyoto Prefectural University

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Tanaka Keiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kubo A

  Laboratory Of Genetic Engineering Faculty Of Agriculture Kyoto Prefectural University

• KURITA Akihiro

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• MORITA Shigeto

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

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