Differential Localization of Tonoplast Intrinsic Proteins on the Membrane of Protein Body Type II and Aleurone Grain in Rice Seeds

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概要

• 論文の詳細を見る

• Tonoplast intrinsic proteins (TIPs) belong to an aquaporin family of proteins that function as water-transport channels. In this study, we isolated and characterized three novel rice cDNAs for OsTIP1, OsTIP2, and OsTIP3 that are homologous to rice γ-TIP cDNA. Northern blot hybridization analyses revealed that rice γ-TIP was expressed in all plant organs. OsTIP1 was expressed in mature seed embryos and during early seed germination. OsTIP2 was expressed exclusively in roots. OsTIP3 was specifically expressed in seeds. These results suggest that the OsTIP1, OsTIP2, and OsTIP3 genes encode discrete, functionally specialized TIPs. Immunocytochemical analysis in rice endosperm cells revealed that rice γ-TIP was localized only on the protein body type II (PB-II) membranes, whereas OsTIP3 was localized on the PB-II and the aleurone grain membranes. Although both the PB-II and the aleurone grain are derived from vacuoles, these results suggest that they may be derived from different types of vacuoles.

• 社団法人 日本農芸化学会の論文
• 2004-08-23

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Tanaka K

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Tanaka K

  Tottori Univ. Tottori

• Masumura Takehiro

  Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Takahashi Hideyuki

  Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Japan

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MORITA Shigeto

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MASUMURA Takehiro

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• KITAGAWA Tomoya

  Laboratory of Genetic Engineering, Graduate School of Agriculture, Kyoto Prefectural University

• RAI Mika

  Laboratory of Genetic Engineering, Graduate School of Agriculture, Kyoto Prefectural University

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Masumura T

  Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center

• Rai Mika

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Takahashi Hideyuki

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Tanaka Keiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Koyanagi Takashi

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• MORITA Shigeto

  Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University

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