A Serine Protease in Soybean Seeds That Acts Specifically on the Native α Subunit of β-Conglycinin : PROTEINS, ENZYMES AND METABOLISM

スポンサーリンク

概要

• 論文の詳細を見る

• A proteolytic activity directed against the a subunit of β-conglycinin was detected in resting mature seeds of the soybean [Glycine max (L.) Merrill] cultivar Keburi. The relationship between pH and activity and the effect of protease inhibitors revealed that the enzyme was a neutral/alkaline serine protease. The proteolysis of the a subunit of β-conglycinin yielded a specific product with a molecular weight of about 47,000, as determined by SDS-PAGE, but the enzyme had no activity against the β subunit. The amino acid composition, the molecular weight and the amino-terminal amino acid sequence of the proteolytic product revealed that the action of the enzyme on the a subunit was specific, with cleavage occurring only at the R126-R127 peptide bond of the a subunit. These characteristics of the protease indicate that the enzyme is a novel protease that has not previously been recognized in soybean seeds.

• 日本植物生理学会の論文

著者

• Morita Shigeto

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Fukuda Y

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Morita Y

  Japan Advanced Inst. Of Sci. And Technol. School Of Materials Sci. Ishikawa

• Morita Y

  Kyoto Univ. Kyoto‐shi

• Yamada Makiko

  San-ei Gen F.f.i. Inc.

• Morita S

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• MORITA Shimpei

  Central Research Institute, Fuji Oil Co.

• FUKASE Masami

  Central Research Institute, Fuji Oil Co.

• MORITA Yuhei

  Central Research Institute, Fuji Oil Co.

• YAMAGUCHI Masami

  Central Research Institute, Fuji Oil Co.

• FUKUDA Yoichi

  Central Research Institute, Fuji Oil Co.

• HOSHINO Kumiko

  Central Research Institute, Fuji Oil Co.

• Morita Y

  Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.

• Yahiro Masatoshi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Morita Yoshikazu

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Yamaguchi Masayo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Fukase Masami

  Central Research Institute Fuji Oil Co.

• Hoshino Kumiko

  Central Research Institute Fuji Oil Co.

• Yamaguchi M

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamaguchi M

  Murata Manufacturing Co. Ltd.

• Yamaguchi Magoichi

  Research Institute Nihon Shokuhin Kako Co. Ltd.

関連論文

• The Fragments of Bovine High Molecular Weight Kininogen Promote Osteoblast Proliferation In Vitro
• Cystatin C in Milk Basic Protein (MBP) and Its Inhibitory Effect on Bone Resorption in Vitro(Food & Nutrition Science)
• Milk Basic Protein Promotes Bone Formation and Suppresses Bone Resorption in Healthy Adult Men
• Controlled Trial of the Effects of Milk Basic Protein (MBP) Supplementation on Bone Metabolism in Healthy Adult Women
• Malonylated Anthocyanins from Bulbs of Red Onion, Allium cepa L.
• Purification and Characterization of a Novel Fungal α-Glucosidase from Mortierella alliacea with High Starch-hydrolytic Activity(Biochemistry & Molecular Biology)
• Hydrolysis of Synthetic Substrate, L-Pyroglutamyl p-Nitroanilide is Catalyzed Solely by Pyroglutamyl Aminopeptidase I in Rat Liver Cytosol(Biochemistry/Molecular Biology)
• Structural Determination of Unknown Subsidiary Colors in Commercial Food Red Nos. 2 and 102
• Structural Determination of Unknown Subsidiary Colors in Food Yellow No. 5(Sunset Yellow FCF)
• Thermal Stability of Individual Molecular Species of Soybean β-Conglycinin
• Substrate Specificity of a Novel Serine Protease from Soybean [Glycine max (L.) Merrill]
• Purification, Characterization, and Crystallization of Single Molecular Species of β-Conglycinin from Soybean Seeds
• Partial Purification and Characterization of a Novel Soybean Protease Which Is Inhibited by Kunitz and Bowman-Birk Trypsin Inbititors
• A Serine Protease in Soybean Seeds That Acts Specifically on the Native α Subunit of β-Conglycinin : PROTEINS, ENZYMES AND METABOLISM
• Purification and Characterization of Rice Lipoxygenase Component 3 from Embryos(Biological Chemistry)
• Changes in Lipoxygenase Components of Rice Seedlings during Germination
• Highly efficient production of human interferon-α by transgenic cultured rice cells
• Production of biologically active human interferon-α in transgenic rice
• A Novel Vesicle Derived Directly from Endoplasmic Reticulum is Involved in the Transport of Vacuolar Storage Proteins in Rice Endosperm
• Differential Localization of Tonoplast Intrinsic Proteins on the Membrane of Protein Body Type II and Aleurone Grain in Rice Seeds
• Preparation of an Endosperm Protoplasts from a Dwarf Rice Variety and Transient Expression of Green-Fluorescent Protein
• Separation of Specific Cells Using a Laser Micro-dissection System and Detection of mRNA in Developing Rice Endosperm Tissue
• Hosetsu-dwarf ; Useful Rice for Molecular Biological Research on Plant Seed Development
• A NOVEL cis-ELEMENT RESPONSIVE TO OXIDATIVE STRESS EXISTING IN ANTIOXIDATIVE DEFENSE GENES IN RICE
• Separation of specific cells from the rice differentiating endosperm using a laser micro-dissection system and detection of expressing genes
• MOLECULAR CHARACTERIZATION OF RECOMBINANT DEHYDROASCORBATE REDUCTASE FROM RICE, AND ITS INTRODUCTION TO TOBACCO CHLOROPLASTS BY GENE MANIPULATION
• THE EXPRESSION OF RICE CHLOROPLASTIC ASCORBATE PEROXIDASE IN RESPONSE TO LIGHT
• rER Differentiation and Localization in Rice Endosperm Cells Involved in Protein Storage
• Induction of Rice Cytosolic Ascorbate Peroxidase mRNA by Oxidative Stress;the Involvement of Hydrogen Peroxide in Oxidative Stress Signalling
• cDNA CLONING AND CHARACTERIZATION OF IRON-SUPEROXIDE DISMUTASE IN RICE
• INDUCTION OF RICE CYTOSOLIC ASCORBATE PEROXIDASE BY OXIDATIVE STRESS
• Optically Active Cyclohexene Derivative as a New Antisepsis Agent : An Efficient Synthesis of Ethyl (6R)-6-[N-(2-Chloro-4-fluorophenyl)-sulfamoyl] cyclohex-1-ene-1-carboxylate (TAK-242)
• Optically Active Antifungal Azoles. XIII.Synthesis of Stereoisomers and Metabolites of 1-[(lR, 2R)-2-(2, 4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1, 2, 4-triazol-1-yl)propyl]-3-[4-(1H-1-tetrazolyl)phenyl]-2-imidazolidinone (TAK-456)
• Optically Active Antifungal Azoles. XII. Synthesis and Antifungal Activity of the Water-Soluble Prodrugs of l-[(lR, 2R)-2-(2, 4-Difluorophenyl)-2-hydroxy-l-methyl-3-(1H-1, 2, 4-triazol-1-yl)propyl]3-[4-(lH-1-tetrazolyl)phenyl]-2-imidazolidinone
• Optically Active Antifungal Azoles. XI. An Alternative Synthetic Route for 1-[(1R, 2R)-2-(2,4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-3-[4-(1H-1-tetrazolyl)phenyl]-2-imidazolidinone (TAK-456) and Its Analog
• Optically Active Antifungal Azoles. X. Synthesis and Antifungal Activity of N-[4-(Azolyl)phenyl]- and N-[4-(Azolylmethyl)phenyl]-N'-[(1R, 2R)-2-(2,4-difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-azolones
• Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124
• Molecular Cloning and Characterization of a cDNA for an Iron-Superoxide Dismutase in Rice (Oryza sativa L.)
• The Crystal Structure of Lipase II from Rhizopus niveus at 2.2 Å Resolution
• Purification, Characterization, and Crystallization of Two Types of Lipase from Rhizopus niveus
• Pyroglutamyl Aminopeptidase I, as a Drug Metabolizing Enzyme, Recognizes Xenobiotic Substrates Containing L-2-Oxothiazolidine-4-carboxylic Acid(Biochemistry/Molecular Biology)
• Gene Cloning and Expression of Cytosolic Glutathione Reductase in Rice : Oryza Sativa L.
• GENE STRUCTURE AND SUBCELLULAR LOCALIZATION OF RICE CYTOSOLIC GLUTATHIONE REDUCTASE
• REDOX RESPONSE OF cis-ACTIVITY OF A NOVEL cis-ELEMENT-LIKE SEQUENCE EXISTING IN ANTIOXIDATIVE DEFFENSE GENES IN RICE
• Molecular Cloning and Characterization of a cDNA for Plastidic Copper/Zinc-Superoxide Dismutase in Rice (Oryza sativa L.)
• The Role of SH and S-S Group in Bacillus cereus β-Amylase
• Two Sulfhydryl Groups Near the Active Site of Soybean β-Amylase
• Interaction of Native and SH-Modified β-Amylase of Soybean with Cyclohexadextrin and Maltose
• Substantially Complete Removal of the 34 kDa Allergenic Soybean Protein, Gly m Bd 30 K, from Soy Milk of a Mutant Lacking the α- and α'-Subunits of Conglycinin
• Optically Active Antifungal Azoles. IX. An Alternative Synthetic Route for 2-[(1R, 2R)-2-(2,4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-4-[4-(2,2,3,3-tetrafluoropropoxy)phenyl]-3(2H, 4H)-1,2,4-triazolone and Its Analogs
• Optically Active Antifungal Azoles. VIII. Synthesis and Antifungal Activity of 1-[(1R, 2R)-2-(2,4-Difluoro- and 2-Fluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-3-(4-substituted phenyl)-2(1H, 3H)-imidazolones and 2-Imidazolidinones
• Optically Active Antifungal Azoles. VII. Synthesis and Antifungal Activity of Stereoisomers of 2-[(1R, 2R)-2-(2,4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-4-[4-(2,2,3,3-tetrafluoropropoxy)phenyl]-3(2H, 4H)-1,2,4-triazolone (TAK-
• Optically Active Antifungal Azoles. VI. Synthesis and Antifungal Activity of N-[(1R, 2R)-2-(2,4-Difluorophenyl)-2-hydroxy-1-methyl-3-(1H-1,2,4-triazol-1-yl)propyl]-N'-(4-substituted phenyl)-3(2H, 4H)-1,2,4-triazolones and 5(1H, 4H)-tetrazolones
• Effects of Medium-Chain Fatty Acids on Intracellular Calcium Levels and the Cytoskeleton in Human Intestinal (Caco-2) Cell Monolayers
• PRODUCTION AND ANALYSIS OF TRANSGENIC TOBACCO PLANTS THAT OVEREXPRESS SUPEROXIDE DISMUTASE AND ASCORBATE PEROXIDASE
• Tableting of Coated Particles. II. Influence of Particle Size of Pharmaceutical Additives on Protection of Coating Membrane from Mechanical Damage during Compression Process
• Tableting of Coated Particles. I. Small Particle Size Chitosan as an Agent Protecting Coating Membrane from Mechanical Damage of Compression Force
• Spasmolytic Effect of Efonidipine Hydrochloride in Isolated Canine Coronary Artery : Comparison with the Effects of Nifedipine and Nisoldipine
• Anthocyanins in The Dark Purple Anthers of Tulipa gesneriana : Identification of Two Novel Delphinidin 3-O-(6-O-(Acetyl-α-Rhamnopyranosyl)-β-Glucopyranosides)
• Antimutagenicity of Deacylated Anthocyanins in Purple-fleshed Sweetpotato(Food & Nutrition Science)
• Antimutagenicity of Sweetpotato (Ipomoea batatas) Roots
• Identification of Cyanidin 3-O-(3", 6"-O-Dimalonyl-β-glucopyranoside) as a Flower Pigment of Chrysanthemum (Dendranthema grandflorum)
• Analysis of the metabolites of the sodium salt of 6-hydroxy-5- (phenylazo) -2-naphthalenesulfonic acid in Sprague-Dawley rat urine
• Effects of Medium-chain Fatty Acids and Their Acylglycerols on the Transport of Penicillin V across Caco-2 Cell Monolayers
• Effects of Benadrostin, a Poly (ADP-Ribose) Polymerase Inhibitor, on Apoptosis of HL-60 Induced by 5-Azacytidine
• The Effects of Corn Peptide Ingestion on Facilitating Alcohol Metabolism in Healthy Men
• MOLECULAR CLONING OF RICE GLUTATHIONE REDUCTASE(GR)cDNAs AND THE EXPPESSION IN RESPONSE TO STRESSES
• MOLECULAR CLONING OF RICE ASCORBATEPEROXIDASE (APX) cDNAS AND THEIR EXPRESSION IN RESPONSE TOSTRESSES
• Ultrastructure of Mature Protein Body in the Starchy Endosperm of Dry Cereal Grain
• Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains
• Effect of Long-Term ‘Corn Peptide’ Ingestion on Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats with Alcohol Loading
• Preparation of Corn Peptide from Corn Gluten Meal and Its Administration Effect on Alcohol Metabolism in Stroke-Prone Spontaneously Hypertensive Rats
• Separation and Identification of Rice Prolamins by Two-Dimensional Gel Electrophoresis and Amino Acid Sequencing
• Heteroplasmy and expression of mitochondrial genes in alloplasmic and euplasmic wheat
• Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants
• Separation and Identification of Rice Prolamins by Two-Dimensional Gel Electrophoresis and Amino Acid Sequencing
• Combined Effects of Dietary Protein and Fat on Lipid Metabolism in Rats.
• Substrate Specificity of a Novel Serine Protease from Soybean (Glycine max (L.) Merrill).

もっと見る 閉じる

スポンサーリンク