Effects of Medium-Chain Fatty Acids on Intracellular Calcium Levels and the Cytoskeleton in Human Intestinal (Caco-2) Cell Monolayers

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概要

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• The effects of medium-chain fatty acids (MCFA) on intracellular calcium (Ca^<2+>) levels and actin filaments in the Caco-2 monolayer were investigated. A site-dependent increase in intracellular Ca^<2+> levels caused by decanoic acid (C10) at 13 mM was observed by confocal laser scanning microscopy. The area in which the intracellular Ca^<2+> levels was increased was measured by image analysis, and increased to 11% of the total area of the monolayer within 1 minute. This was maintained for 5 minutes, and decreased thereafter. The other MCFAs did not significantly increase the intracellular Ca^<2+> levels. Obvious morphological changes of actin filaments were induced by only C10 among C8-C14. The area in which actin filaments were depleted was also quantified, and the increase in area became significant after 40 minutes. The area of the actin-depleted spot corresponded to the area occupied by 5 to 10 cells as well as that in which the intracellular Ca^<2+> level was increased. The effectiveness of only C10 suggested that the mechanism of the absorption enhancement by C10 would be different from that by the other MCFAs, or that C10 has some additional physiological functions although the mechanism of the enhancement is the same as for the other MCFAs.

• 社団法人日本農芸化学会の論文
• 2001-04-23

著者

• Kimura Yoshinobu

  The International Center For Biotechnology Osaka University

• Kimura Y

  Department Of Bioresource Science Faculty Of Agriculture Tottori University

• Yamada Makiko

  San-ei Gen F.f.i. Inc.

• ADACHI Shuji

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• Hosoda Yasuo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Kimura Y

  Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University

• Kimura Y

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Adachi S

  Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.

• Adachi S

  Kyoto Univ. Graduate School Of Medicine Kyoto Jpn

• Adachi Shuji

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• Adachi Shuji

  School Of Food And Nutritional Sciences University Of Shizuoka

• Adachi Shuji

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• MATSUNO Ryuichi

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• SHIMA Motohiro

  Division of Food Science and Technology, Graduate School of Agriculture, Kyoto University

• Yamaguchi Masayo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Shima Motohiro

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• KIMURA Yukitaka

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• NAGANO Hiroshi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Yamaguchi M

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Matsuno R

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• Hosoda Yoshisuke

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Kimura Yukitaka

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• Yamaguchi M

  Murata Manufacturing Co. Ltd.

• Kimura Y

  Mukogawa Women's Univ. Nishinomiya Jpn

• Kimura Y

  Department Of Biological And Environmental Chemistry Tottori University

• Adachi Shuji

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yamaguchi Magoichi

  Research Institute Nihon Shokuhin Kako Co. Ltd.

• Matsuno Ryuichi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Nagano Hiroshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

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