The Promoter of the Gene for Glutamine Synthetase from Rice Shows Organ-Specific and Substrate-Induced Expression in Transgenic Tobacco Plants

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概要

• 論文の詳細を見る

• A 2-kb fragment from the 5'-flanking region of the RGS-28 gene, which encodes the cytosolic glutamine synthetase in Oryza sativa L., was fused to a β-glucuronidase (GUS) reporter gene and introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. The promoter was predominantly active in the leaves of transgenic plants, as it is in authentic rice plants. The promoter also responded to externally applied ammonium ions. It is suggested that the cis-acting regulatory elements responsible for the recognition of the leaf as a site of synthesis and of ammonia, a substrate for glutamine synthetase, are located within a 2-kb region of the promoter.

• 日本植物生理学会の論文

著者

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• SAKAMOTO Atsushi

  Natl. Inst. Basic Biol.

• Takeba Go

  Laboratory of Applied Biology, Faculty of Life Science, Kyoto Prefectural University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Life Science Kyoto Prefectural University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Life Science Kyoto Prefecture University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Living Science Kyoto Prefecture University

• Takeba Go

  Kyoto Pref. Univ.

• Takeba Go

  Laboralory Of Applied Biology Faculty Of Living Science Kyoto Prefectural University

• Sakamoto Atsushi

  National Institute For Basic Biology:(present Address)department Of Mathematical And Life Sciences G

• Tanaka Katsuyuki

  Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.

• Sakamoto A

  Natl. Inst. Basic Biol.

• Kozaki Akiko

  Laboratory of Applied Botany, Faculty of Agriculture, Kyoto University

• Sakamoto Atsushi

  Laboratory of Biochemistry, Faculty of Agriculture, Kyoto Prefectural University

• Kozaki Akiko

  Laboratory Of Applied Botany Faculty Of Agriculture Kyoto University

• Tanaka K

  Dept. Biol. Sci. Fac. Sci. Hiroshima Univ.

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Sakamoto Atsushi

  Minamata Research Center Chisso Corporation

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