The Promoter of the Gene for Plastidic Glutamine Synthetase (GS2) from Rice Is Developmentally Regulated and Exhibits Substrate-Induced Expression in Transgenic Tobacco Plants

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概要

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• A 1.3-kb fragment from the 5'-flanking region of the RGS-38 gene, which encodes the plastidic glutamine synthetase in Oryza sativa L., was fused to a β-glucuronidase (GUS) reporter gene and introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. The promoter directed GUS expression, both in leaves and in roots, and the expression of GUS was regulated by light. The GUS activity was high in the mature leaves of the transgenic tobacco plants, in marked contrast to the activity of the GS1 promoter. The GS2 promoter also responded to externally applied ammonia, as is the case for the GS1 promoter. These results suggest that the cis-acting regulatory elements that control the response to ammonia, a substrate for glutamine synthetase, are located within a 1.3-kb region of the promoter.

• 日本植物生理学会の論文

著者

• SAKAMOTO Atsushi

  Natl. Inst. Basic Biol.

• Takeba Go

  Laboratory of Applied Biology, Faculty of Life Science, Kyoto Prefectural University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Life Science Kyoto Prefectural University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Life Science Kyoto Prefecture University

• Takeba Go

  Laboratory Of Applied Biology Faculty Of Living Science Kyoto Prefecture University

• Takeba Go

  Kyoto Pref. Univ.

• Takeba Go

  Laboralory Of Applied Biology Faculty Of Living Science Kyoto Prefectural University

• Sakamoto Atsushi

  National Institute For Basic Biology:(present Address)department Of Mathematical And Life Sciences G

• Sakamoto A

  Natl. Inst. Basic Biol.

• Kozaki Akiko

  Laboratory of Applied Botany, Faculty of Agriculture, Kyoto University

• Sakamoto Atsushi

  Laboratory of Biochemistry, Faculty of Agriculture, Kyoto Prefectural University

• Kozaki Akiko

  Laboratory Of Applied Botany Faculty Of Agriculture Kyoto University

• Sakamoto Atsushi

  Minamata Research Center Chisso Corporation

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