Rapid Decrease in the Glutamine Synthetase Activity during Imbibition of Thermodormant New York Lettuce Seeds
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A high glutamine synthetase (GS) activity was detected in dry seeds of New York lettuce but it decreased rapidly during imbibition at 35℃. The decrease in GS activity was remarkable at 35℃, but not so at 45℃ or at 25℃. The activity of extracted GS was relatively constant even at 35℃. The decrease in GS activity stopped immediately after the addition of cycloheximide (CH). This suggests the presence of a GS-inactivating system in the seeds. The amount of ammonia increased abruptly during the early stage of imbibition at 35℃, suggesting the blockage of ammonia-assimilation at high temperature. The GS activity that decreased to a low level during imbibition at 35℃ for 12 h increased again during the subsequent imbibition at low temperature (15℃) before the break of thermodormancy. A higher GS activity was found in the embryonic axes than in the cotyledons. Partial purification of GS showed that lettuce seed GS was eluted as a single peak on Biogel A1.5m or DEAE-Sephacel (mol wt: 4.4 × 10^5). Thus the thermodormancy of New York lettuce seeds may be related to inactivation of GS during imbibition at high temperatures, and the activity of GS in the embryonic axes may control the germination of New York lettuce seeds through the regulation of glutamine formation.
- 日本植物生理学会の論文
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