1. Genetic Engineering of Cold-Tolerant Tomato via Glycinebetaine Biosynthesis
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概要
- 論文の詳細を見る
Tomato (Lycopersicon esculentum Mill.) plants, which normally do not accumulate glycinebetaine (GB), are susceptible to chilling stress. Exposure to temperatures below 10℃ causes various symptoms of injury, and greatly decreases fruit set (i.e., the number of fruits per plants) in most cultivars. Conventional breeding efforts have failed to produce cultivars with satisfactory cold tolerance. Therefore, we have transformed tomato (cv. Moneymaker) with the cod A. gene of Arthrobacter globiformis, which encodes choline oxidase to catalyze the conversion of choline to GB. These transgenic plants express the cod A gene and synthesize choline oxidase, while accumulating up to 0.23μmol of GB per gram fresh weight in their leaves. During various phases of growth and development, from seed germination to fruit production, the GB-accumulating plants are more tolerant of chilling stress than their wild-type counterparts. Moreover, at the reproductive stage, our transgenic tomato plants yield, on average, 30% more fruit after an episode of chilling stress. We demonstrate here that introducing the biosynthetic pathway of GB into tomato via metabolic engineering is an effective strategy for improving fruit production under cold-stress conditions.
- 低温生物工学会の論文
- 2003-12-30
著者
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Murata N
National Institute For Basic Biology
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MURATA Norio
National Institute of Basic Biology (NIBB)
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Murata Norio
National Institute For Basic Biology
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Jeknic Zoran
Department Of Horticulture Als 4017 Oregon State University
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PARK Eung-Jun
Department of Horticulture, ALS 4017, Oregon State University
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SAKAMOTO Atsushi
National Institute for Basic Biology
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DeNOMA Jeanine
Department of Horticulture, ALS 4017, Oregon State University
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CHEN Tony
Department of Horticulture, ALS 4017, Oregon State University
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SAKAMOTO Atsushi
Natl. Inst. Basic Biol.
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Sakamoto Atsushi
National Institute For Basic Biology:(present Address)department Of Mathematical And Life Sciences G
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Sakamoto A
Natl. Inst. Basic Biol.
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Chen Tony
Department Of Horticulture Als 4017 Oregon State University
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Chen Tony
Department Of Horticulture 4017 Ag And Life Sciences
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Jeknic Zoran
Department Of Horticulture 4017 Agricultural And Life Sciences Oregon State University
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Murata Norio
Natl.inst.basic Biol.
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Park Eung-jun
Department Of Horticulture Als 4017 Oregon State University
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Denoma Jeanine
Department Of Horticulture Als 4017 Oregon State University:(present Address)department Of Forest Sc
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Sakamoto Atsushi
Minamata Research Center Chisso Corporation
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Murata Norio
Department Of Molecular Biomechanics Thegraduate University For Advanced Studies National Institute
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DeNoma Jeanine
National Institute for Basic Biology
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