Synechocystis PCC6803 Mutants Defective in Destruction of Fatty Acids

概要

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Two mutants, which were defective in desaturation of fatty acids, were isolated from the cyanobacterium, Synechocystis PCC6803, by treating wild-type cells with ethyl methanesulfonate. They were designated as Fad6 and Fad12. Fad6 was defective in desaturation at the Δ^6 position of C_<18> fatty acids esterified to the sn-1 position of glycerol moiety in monogalactosyl diacylglycerol and digalactosyl diacylglycerol but not sulfoquinovosyl diacylglycerol and phosphatidylglycerol. This observation suggests that the desaturation affected in this mutant was specific to the sn-glycerol position, the chain length and the polar head group. Fad12 was defective in desaturation at the Δ^<12> position of C_<18> fatty acids at the sn-1 position of glycerol moiety in all the lipid classes. This observation suggests that the desaturation affected in this mutant was specific with respect to the sn-glycerol position and the chain length of fatty acids, but not to the polar head group of the lipids. At 22℃ Fad6 grew as fast as the wild type, but the growth rate of Fad12 was much lower than that of wild type. At 34℃, on the other hand, the mutants and wild type grew at about the same rates.
日本植物生理学会の論文